Simultaneous enantiomeric analysis of pharmacologically active compounds in environmental samples by chiral LC–MS/MS with a macrocyclic antibiotic stationary phase

Dolores Camacho-Muñoz, Barbara Kasprzyk-Hordern

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23 Citations (Scopus)

Abstract

This paper presents a multi-residue method for direct enantioselective separation of chiral pharmacologically active compounds in environmental matrices. The method is based on chiral liquid chromatography and tandem mass spectrometry detection. Simultaneous chiral discrimination was achieved with a macrocyclic glycopeptide-based column with antibiotic teicoplanin as a chiral selector working under reverse phase mode. For the first time, enantioresolution was reported for metabolites of ibuprofen: carboxyibuprofen and 2-hydroxyibuprofen with this chiral stationary phase. Moreover, enantiomers of chloramphenicol, ibuprofen, ifosfamide, indoprofen, ketoprofen, naproxen and praziquantel were also resolved. The overall performance of the method was satisfactory in terms of linearity, precision, accuracy and limits of detection. The method was successfully applied for monitoring of pharmacologically active compounds at enantiomeric level in influent and effluent wastewater and in river water. In addition, the chiral recognition and analytical performance of the teicoplanin-based column was critically compared with that of the α1-acid glycoprotein chiral stationary phase.

Original languageEnglish
Pages (from-to)94-108
Number of pages15
JournalJournal of Mass Spectrometry
Volume52
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017

Keywords

  • acid-glycoprotein
  • chiral analysis
  • river water
  • teicoplanin
  • wastewater

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