Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Chetna Taneja; Jerin Geogy George; Stella Corsetti; Philip Wijesinghe; Graham D. Bruce; Maarten F. Zwart; Shanti Bhattacharya; Kishan Dholakia

doi:10.1364/BOE.538253

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinghe, Graham D. Bruce, Maarten F. Zwart, Shanti Bhattacharya, Kishan Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

5 Citations (SciVal)

Abstract

The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 µm in phantom samples maintaining a transverse size of 5 µm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.

Original language	English
Pages (from-to)	6183-6197
Number of pages	15
Journal	Biomedical Optics Express
Volume	15
Issue number	11
Early online date	4 Oct 2024
DOIs	https://doi.org/10.1364/BOE.538253
Publication status	Published - 1 Nov 2024

Data Availability Statement

The research data underpinning this publication can be accessed at [44].

Acknowledgements

We thank Dr. Pierce Mullen, Eleonora Gagliardi from the School of Psychology and
Neuroscience, University of St Andrews for providing labelled zebrafish larvae samples.

Funding

Engineering and Physical Sciences Research Council (EP/R004854/1); HORIZON EUROPE Innovative Europe (EC-GA863203); Australian Research Council (FL210100099); Scheme for Promotion of Academic and Research Collaboration (SPARC/2018-2019/P796/SL); Biotechnology and Biological Sciences Research Council (BB/T006560/1); Royal Commission for the Exhibition of 1851 (Research fellowship).

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.538253Licence: CC BY

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title = "Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy",

abstract = "The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 µm in phantom samples maintaining a transverse size of 5 µm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.",

author = "Chetna Taneja and George, \{Jerin Geogy\} and Stella Corsetti and Philip Wijesinghe and Bruce, \{Graham D.\} and Zwart, \{Maarten F.\} and Shanti Bhattacharya and Kishan Dholakia",

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AU - George, Jerin Geogy

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AU - Wijesinghe, Philip

AU - Bruce, Graham D.

AU - Zwart, Maarten F.

AU - Bhattacharya, Shanti

AU - Dholakia, Kishan

PY - 2024/11/1

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N2 - The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 µm in phantom samples maintaining a transverse size of 5 µm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.

AB - The Bessel beam (BB) has found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer-generated phase element for generating a sidelobe suppressed Bessel beam (SSBB). We then progress to perform a comparison of biological imaging using SSBB to standard BB in a light-sheet geometry. The SSBB peak intensity is more than an order of magnitude higher than the first sidelobe. In contrast to a standard BB light-sheet, an SSBB does not need deconvolution. The SSBB propagates to depths exceeding 400 µm in phantom samples maintaining a transverse size of 5 µm. Finally, we demonstrate the advantage of using an SSBB light-sheet for biological applications by imaging fixed early-stage zebrafish larvae. In comparison to the standard BB, we observe a two-fold increase in contrast-to-noise ratio (CNR) when imaging the labelled cellular eye structures and the notochords. Our results provide an effective approach to generating and using SSBB light-sheets to enhance contrast for one-photon light-sheet microscopy.

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Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

Abstract

Data Availability Statement

Acknowledgements

Funding

ASJC Scopus subject areas

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