This paper attempts to explore the dynamic behaviour of traditional Dieh-Dou timber structure under different combinations of structural forms and vertical loads. Using time-history record (TCU 084) from the Chi-Chi earthquake, two semi full-scale specimens (Symmetric and Asymmetric) were tested. Results showed that the Symmetric specimen tends to be damaged more easily and faster than the Asymmetric one. Damage pattern generally begins from the bottom Dou members and subsequently spreading upwards to the upper Dou, horizontal Gong members and adjoining Shu members. Friction force between the contact surfaces is crucial towards the maintenance of overall structure. Increase vertical loadings have significant effect on the natural frequencies and global stiffness of the structure. Using the Single-Degree-Of-Freedom (SDOF) system, the derived stiffness is generally in good agreement with the dynamic results of both forms. This study suggests that the effects of increasing vertical loadings should be taken into consideration for future evaluation.