Sensitive fluorescence-based detection of magnetic field effects in photoreactions of flavins

Emrys Evans, Jing Li, Jonathan Storey, Kiminori Maeda, Kevin Henbest, Charlotte Dodson, Peter Hore, Stuart Mackenzie, Christiane Timmel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Magnetic field effect studies have been conducted on a variety of flavin-based radical pair systems chosen to model the magnetosensitivity of the photoinduced radical pairs found in cryptochrome flavoproteins. Cryptochromes are blue-light photoreceptor proteins which are thought to mediate avian magnetoreception, an hypothesis supported by recent in vitro observations of magnetic field-dependent reaction kinetics for a light-induced radical pair in a cryptochrome from the plant Arabidopsis thaliana. Many cryptochromes are difficult to express in large quantities or high concentrations and are easily photodegraded. Magnetic field effects are typically measured by spectroscopic detection of the transient radical (pair) concentrations. Due to its low sensitivity, single-pass transient absorption spectroscopy can be of limited use in such experiments and much recent work has involved development of other methodologies offering improved sensitivity. Here we explore the use of flavin fluorescence as the magnetosensitive probe and demonstrate the exceptional sensitivity of this technique which allows the detection of magnetic field effects in flavin samples at sub-nanomolar concentrations and in cryptochromes.
Original languageEnglish
Pages (from-to)18456-18463
Number of pages8
JournalPhysical Chemistry Chemical Physics
Volume17
Issue number28
DOIs
Publication statusPublished - 4 Jun 2015

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Cryptochromes
Flavins
Magnetic field effects
Fluorescence
fluorescence
magnetic fields
sensitivity
photoreceptors
Flavoproteins
absorption spectroscopy
reaction kinetics
Absorption spectroscopy
Reaction kinetics
methodology
proteins
probes
Magnetic fields
4,6-dinitro-o-cresol
Proteins

Cite this

Sensitive fluorescence-based detection of magnetic field effects in photoreactions of flavins. / Evans, Emrys; Li, Jing; Storey, Jonathan; Maeda, Kiminori; Henbest, Kevin; Dodson, Charlotte; Hore, Peter; Mackenzie, Stuart; Timmel, Christiane.

In: Physical Chemistry Chemical Physics, Vol. 17, No. 28, 04.06.2015, p. 18456-18463.

Research output: Contribution to journalArticle

Evans, E, Li, J, Storey, J, Maeda, K, Henbest, K, Dodson, C, Hore, P, Mackenzie, S & Timmel, C 2015, 'Sensitive fluorescence-based detection of magnetic field effects in photoreactions of flavins', Physical Chemistry Chemical Physics, vol. 17, no. 28, pp. 18456-18463. https://doi.org/10.1039/C5CP00723B
Evans, Emrys ; Li, Jing ; Storey, Jonathan ; Maeda, Kiminori ; Henbest, Kevin ; Dodson, Charlotte ; Hore, Peter ; Mackenzie, Stuart ; Timmel, Christiane. / Sensitive fluorescence-based detection of magnetic field effects in photoreactions of flavins. In: Physical Chemistry Chemical Physics. 2015 ; Vol. 17, No. 28. pp. 18456-18463.
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