Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection

Pavel Zhurauski, Sunil Arya, Pawan Jolly, Christian Tiede, Darren Tomlinson, Paul Ko-Ferrigno, Pedro Estrela

Research output: Contribution to journalArticle

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Abstract

A novel Affimer-functionalised interdigitated electrode-based capacitive biosensor platform was developed for detection and estimation of Her4, a protein tumour biomarker, in undiluted serum. An anti-Her4 Affimer with a C-terminal cysteine was used to create the bio-recognition layer via self-assembly on gold interdigitated electrodes for the sensor fabrication. Electrochemical impedance spectroscopy (EIS) in the absence of redox markers was used to evaluate the sensor performance by monitoring the changes in capacitance. The Affimer sensor in buffer and in undiluted serum demonstrated high sensitivity with a broad dynamic range from 1 pM to 100 nM and a limit of detection lower than 1 pM both in buffer and in serum. Furthermore, the Affimer sensor demonstrated excellent specificity with negligible interference from serum proteins, suggesting resilience to non-specific binding. The sensing ability of the present Affimer sensor in spiked undiluted serum suggests its potential for a new range of Affimer-based sensors. The fabricated Affimer sensor can thus be further adapted with other probes having affinities to other biomarkers for a new range of biosensors.
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiosensors and Bioelectronics
Volume108
Early online date21 Feb 2018
DOIs
Publication statusPublished - 15 Jun 2018

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Biosensing Techniques
Tumor Biomarkers
Biosensors
Electrodes
Proteins
Sensors
Serum
Buffers
Dielectric Spectroscopy
Gold
Oxidation-Reduction
Cysteine
Limit of Detection
Blood Proteins
Biomarkers
Electrochemical impedance spectroscopy
Self assembly
Capacitance
Fabrication
Monitoring

Keywords

  • Biosensor
  • Affimer
  • Impedance
  • Capacitance
  • serum
  • Her4

Cite this

Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection. / Zhurauski, Pavel; Arya, Sunil; Jolly, Pawan; Tiede, Christian; Tomlinson, Darren; Ko-Ferrigno, Paul; Estrela, Pedro.

In: Biosensors and Bioelectronics, Vol. 108, 15.06.2018, p. 1-8.

Research output: Contribution to journalArticle

Zhurauski, Pavel ; Arya, Sunil ; Jolly, Pawan ; Tiede, Christian ; Tomlinson, Darren ; Ko-Ferrigno, Paul ; Estrela, Pedro. / Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection. In: Biosensors and Bioelectronics. 2018 ; Vol. 108. pp. 1-8.
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