Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes

Maria Weber; Namiko Yamada; Xue Tian; Steven D. Bull; Masafumi Minoshima; Kazuya Kikuchi; Amanda B. Mackenzie; Tony D. James

doi:10.3389/fchem.2020.00039

Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes

Maria Weber, Namiko Yamada, Xue Tian, Steven D. Bull, Masafumi Minoshima, Kazuya Kikuchi, Amanda B. Mackenzie, Tony D. James

Osaka University

Research output: Contribution to journal › Article › peer-review

19 Citations (SciVal)

Abstract

The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO⁻), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO⁻ and a distinctive organelle targeting group. The study of ONOO⁻ generation in a particular organelle will allow more precise disease profiling. Hence, targeting groups for the mitochondria, lysosome and endoplasmic reticulum were introduced into a coumarin scaffold. The three ratiometric probes displayed sensitive and selective detection of ONOO⁻ over other ROS species. All three coumarin probes were evaluated in murine RAW264.7 macrophages for detection of basal and stimulated ONOO⁻ formation.

Original language	English
Article number	39
Journal	Frontiers in Chemistry
Volume	8
DOIs	https://doi.org/10.3389/fchem.2020.00039
Publication status	Published - 20 Feb 2020

Funding

MW would like to thank the EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies (EP/ L016354/1) for providing a PhD studentship. SB would like thank the Royal Society for funding an International Exchange (IEC\R3\183068). MM would like to thank the JSPS for Grant-in-Aid for Scientific Research (16K01933). KK would like to thank the JSPS for Grand in Aid for Research (25220207 and 18H03935) and Innovative Areas Frontier Research on Chemical Communications (17H06409). TJ wishes to thank the Royal Society for a Wolfson Research Merit Award. Characterization facilities were provided through the Material and Chemical Characterization Facility (MC2) at the University of Bath (http://go.bath.ac.uk/mc2) and at Osaka University.

Keywords

fluorescence
inflammation
molecular probe
peroxynitrite
reactive oxygen species

ASJC Scopus subject areas

General Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3389/fchem.2020.00039Licence: CC BY

Cite this

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title = "Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes",

abstract = "The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO−), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO− and a distinctive organelle targeting group. The study of ONOO− generation in a particular organelle will allow more precise disease profiling. Hence, targeting groups for the mitochondria, lysosome and endoplasmic reticulum were introduced into a coumarin scaffold. The three ratiometric probes displayed sensitive and selective detection of ONOO− over other ROS species. All three coumarin probes were evaluated in murine RAW264.7 macrophages for detection of basal and stimulated ONOO− formation.",

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AU - Minoshima, Masafumi

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AU - James, Tony D.

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Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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Cite this

Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Equipment

Microplate reader BMG Labtech CLARIOstar

Cite this