Projects per year
Results: In batch fermentations on the 2-L scale, a lipid concentration of 14.6 g L−1 and productivity of 0.11 g L−1 h−1 were achieved, which was confirmed at 50 L (15.8 g L−1; 0.10 g L−1 h−1). The maximum lipid production rate was 0.33 g L−1 h−1 (daily average), but the substrate uptake rate decreased with oligosaccharide chain length. To produce 1 kg of dry yeast biomass containing up to 43% (w/w) lipids, 5.2 kg of the glucose syrup was required, with a lipid
yield of up to 0.21 g g−1 consumed saccharides. In semi-continuous operation, for the first time, an oleaginous yeast was cultured for over 2 months with a relatively stable lipid production rate (around 0.08 g L−1 h−1) and fatty acid profile (degree of fatty acid saturation around 27.6% w/w), and without contamination. On the 250-L scale, comparable results were observed, culminating in the generation of nearly 10 kg lipids with a lipid productivity of 0.10 g L−1 h−1.
Conclusions: The results establish the importance of M. pulcherrima for industrial biotechnology and its suitability to commercially produce a food-grade oil. Further improvements in the productivity are required to make M. pulcherrima lipid production industrial reality, particularly when longer-chain saccharides are involved.
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 665992
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- 1 Finished
Integrated Energy Efficient Microwave and Unique Fermentation Processes for Pilot Scale Production of High Value Chemical from Lignocellulosic Waste
1/03/16 → 31/01/21
Project: Research council
- Department of Chemical Engineering - Professor
- Reaction and Catalysis Engineering research unit (RaCE)
- Centre for Sustainable and Circular Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Bioengineering & Biomedical Technologies (CBio)
- EPSRC Centre for Doctoral Training in Advanced Automotive Propulsion Systems (AAPS CDT)
Person: Research & Teaching, Core staff, Affiliate staff