Self-assembled peptide hydrogels

Eleanor K Johnson

Research output: ThesisDoctoral Thesis

Abstract

The use of low-molecular weight peptide-based hydrogelators (LMWGs) for the immobilisation of enzymes is presented in this thesis. Low-molecular weight hydrogelators are a class of materials which are highly suitable for increasing enzyme lifetimes as they create a suitable biomimetic environment. Immobilised enzymes can be utilised in enzyme fuel cells, providing low-energy conversion routes for chemical processes. The hydrogels also possess tunable properties which allow their structure to be manipulated to give desirable properties.

This work begins with an exploration of dipeptide hydrogelators by investigating the effect of varying salt solutions and concentrations of dipeptide on final hydrogel structures. A wide range of characterisation techniques are employed to provide information about the micro- and macro-structure of the hydrogels.

The creation of dipeptide hydrogel materials via an electrochemical method is explored, which allows the production of nanometre thick, membrane-like materials. These layers are measured using Surface Plasmon Resonance techniques. The electrochemical technique for dipeptide gelation is expanded in later chapters to produce a range of novel materials.

Finally, an exploration into the effect of additives on dipeptide hydrogels is conducted, where the effect of adding chiral molecules is investigated. This provides interesting information regarding the self-assembly processes involved with hydrogelation processes, which has important implications for studying the folding and unfolding processes of peptides.

LanguageEnglish
QualificationPh.D.
Awarding Institution
  • University of Bath
Supervisors/Advisors
  • Cameron, Petra, Supervisor
Award date1 Dec 2011
StatusUnpublished - Dec 2011

Fingerprint

Hydrogels
Dipeptides
Peptides
Immobilized Enzymes
Hydrogel
Biomimetics
Surface plasmon resonance
Gelation
Enzymes
Energy conversion
Self assembly
Macros
Fuel cells
Salts
Molecular weight
Membranes
Microstructure
Molecules

Keywords

  • self-assembly
  • peptide
  • hydrogel
  • enzyme immobilistion

Cite this

Johnson, E. K. (2011). Self-assembled peptide hydrogels

Self-assembled peptide hydrogels. / Johnson, Eleanor K.

2011.

Research output: ThesisDoctoral Thesis

Johnson, EK 2011, 'Self-assembled peptide hydrogels', Ph.D., University of Bath.
Johnson, Eleanor K. / Self-assembled peptide hydrogels. 2011.
@phdthesis{470b717639bf43d899940a0bfa95a311,
title = "Self-assembled peptide hydrogels",
abstract = "The use of low-molecular weight peptide-based hydrogelators (LMWGs) for the immobilisation of enzymes is presented in this thesis. Low-molecular weight hydrogelators are a class of materials which are highly suitable for increasing enzyme lifetimes as they create a suitable biomimetic environment. Immobilised enzymes can be utilised in enzyme fuel cells, providing low-energy conversion routes for chemical processes. The hydrogels also possess tunable properties which allow their structure to be manipulated to give desirable properties.This work begins with an exploration of dipeptide hydrogelators by investigating the effect of varying salt solutions and concentrations of dipeptide on final hydrogel structures. A wide range of characterisation techniques are employed to provide information about the micro- and macro-structure of the hydrogels.The creation of dipeptide hydrogel materials via an electrochemical method is explored, which allows the production of nanometre thick, membrane-like materials. These layers are measured using Surface Plasmon Resonance techniques. The electrochemical technique for dipeptide gelation is expanded in later chapters to produce a range of novel materials.Finally, an exploration into the effect of additives on dipeptide hydrogels is conducted, where the effect of adding chiral molecules is investigated. This provides interesting information regarding the self-assembly processes involved with hydrogelation processes, which has important implications for studying the folding and unfolding processes of peptides.",
keywords = "self-assembly, peptide, hydrogel, enzyme immobilistion",
author = "Johnson, {Eleanor K}",
year = "2011",
month = "12",
language = "English",
school = "University of Bath",

}

TY - THES

T1 - Self-assembled peptide hydrogels

AU - Johnson,Eleanor K

PY - 2011/12

Y1 - 2011/12

N2 - The use of low-molecular weight peptide-based hydrogelators (LMWGs) for the immobilisation of enzymes is presented in this thesis. Low-molecular weight hydrogelators are a class of materials which are highly suitable for increasing enzyme lifetimes as they create a suitable biomimetic environment. Immobilised enzymes can be utilised in enzyme fuel cells, providing low-energy conversion routes for chemical processes. The hydrogels also possess tunable properties which allow their structure to be manipulated to give desirable properties.This work begins with an exploration of dipeptide hydrogelators by investigating the effect of varying salt solutions and concentrations of dipeptide on final hydrogel structures. A wide range of characterisation techniques are employed to provide information about the micro- and macro-structure of the hydrogels.The creation of dipeptide hydrogel materials via an electrochemical method is explored, which allows the production of nanometre thick, membrane-like materials. These layers are measured using Surface Plasmon Resonance techniques. The electrochemical technique for dipeptide gelation is expanded in later chapters to produce a range of novel materials.Finally, an exploration into the effect of additives on dipeptide hydrogels is conducted, where the effect of adding chiral molecules is investigated. This provides interesting information regarding the self-assembly processes involved with hydrogelation processes, which has important implications for studying the folding and unfolding processes of peptides.

AB - The use of low-molecular weight peptide-based hydrogelators (LMWGs) for the immobilisation of enzymes is presented in this thesis. Low-molecular weight hydrogelators are a class of materials which are highly suitable for increasing enzyme lifetimes as they create a suitable biomimetic environment. Immobilised enzymes can be utilised in enzyme fuel cells, providing low-energy conversion routes for chemical processes. The hydrogels also possess tunable properties which allow their structure to be manipulated to give desirable properties.This work begins with an exploration of dipeptide hydrogelators by investigating the effect of varying salt solutions and concentrations of dipeptide on final hydrogel structures. A wide range of characterisation techniques are employed to provide information about the micro- and macro-structure of the hydrogels.The creation of dipeptide hydrogel materials via an electrochemical method is explored, which allows the production of nanometre thick, membrane-like materials. These layers are measured using Surface Plasmon Resonance techniques. The electrochemical technique for dipeptide gelation is expanded in later chapters to produce a range of novel materials.Finally, an exploration into the effect of additives on dipeptide hydrogels is conducted, where the effect of adding chiral molecules is investigated. This provides interesting information regarding the self-assembly processes involved with hydrogelation processes, which has important implications for studying the folding and unfolding processes of peptides.

KW - self-assembly

KW - peptide

KW - hydrogel

KW - enzyme immobilistion

M3 - Doctoral Thesis

ER -