Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

Yueci Wu; Lu-Lu Sun; Hai-Hao Han; Xiao-Peng He; Weiguo Cao; Tony D. James

doi:10.1039/D3SC05010F

Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James

Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, P. R. China
National Center for Liver Cancer, the International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
Department of Chemistry, Shanghai University, Shanghai 200444, P. R. China

Research output: Contribution to journal › Article › peer-review

5 Citations (SciVal)

Abstract

Drug-induced liver injury (DILI) is the most common cause for acute liver failure in the USA and Europe. However, most of DILI cases can recover or be prevented if treatment by the offending drug is discontinued. Recent research indicates that peroxynitrite (ONOO−) can be a potential indicator to diagnose DILI at an early stage. Therefore, the establishment of an assay to detect and track ONOO− in DILI cases is urgently needed. Here, a FRET-based ratiometric nano fluorescent probe CD–N-I was developed to detect ONOO− with high selectivity and excellent sensitivity. This probe consists of carbon dots and a naphthalimide–isatin peroxynitrite sensing system assembled based on electrostatic interactions. Using CD–N-I we were able to detect exogenous ONOO− in live cells and endogenous ONOO− in APAP-induced liver injury of HepG2 cells.

Original language	English
Pages (from-to)	757-764
Number of pages	8
Journal	Chemical Science
Volume	15
Issue number	2
Early online date	14 Dec 2023
DOIs	https://doi.org/10.1039/D3SC05010F
Publication status	Published - 14 Jan 2024

Bibliographical note

Data availability:
The authors confirm that the data supporting the findings of this study are available within the article ESI.

Funding

Y. W. wishes to thank China Scholarship Council, the University of Bath and Shanghai University. T. D. J. wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. H.-H. H. thanks the National Natural Science Foundation of China (No. 22107029, 22377135), the Fundamental Research Projects of Science & Technology Innovation and development Plan in Yantai City (2023JCYJ059), and the Shandong Laboratory Program (SYS202205). X.-P. H. thanks the National Natural Science Foundation of China (No. 21788102, 91853201 and 9185920077).

Funders	Funder number
Fundamental Research Projects of Science & Technology Innovation and development Plan in Yantai City	2023JCYJ059
Shandong Laboratory Program	91853201, 9185920077, SYS202205, 21788102
University of Bath and Shanghai University
Henan Normal University	2020ZD01
University of Bath
National Natural Science Foundation of China	22107029, 22377135
China Scholarship Council

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title = "Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury",

abstract = "Drug-induced liver injury (DILI) is the most common cause for acute liver failure in the USA and Europe. However, most of DILI cases can recover or be prevented if treatment by the offending drug is discontinued. Recent research indicates that peroxynitrite (ONOO−) can be a potential indicator to diagnose DILI at an early stage. Therefore, the establishment of an assay to detect and track ONOO− in DILI cases is urgently needed. Here, a FRET-based ratiometric nano fluorescent probe CD–N-I was developed to detect ONOO− with high selectivity and excellent sensitivity. This probe consists of carbon dots and a naphthalimide–isatin peroxynitrite sensing system assembled based on electrostatic interactions. Using CD–N-I we were able to detect exogenous ONOO− in live cells and endogenous ONOO− in APAP-induced liver injury of HepG2 cells.",

author = "Yueci Wu and Lu-Lu Sun and Hai-Hao Han and Xiao-Peng He and Weiguo Cao and James, {Tony D.}",

note = "Data availability: The authors confirm that the data supporting the findings of this study are available within the article ESI.",

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AU - Wu, Yueci

AU - Sun, Lu-Lu

AU - Han, Hai-Hao

AU - He, Xiao-Peng

AU - Cao, Weiguo

AU - James, Tony D.

N1 - Data availability: The authors confirm that the data supporting the findings of this study are available within the article ESI.

PY - 2024/1/14

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AB - Drug-induced liver injury (DILI) is the most common cause for acute liver failure in the USA and Europe. However, most of DILI cases can recover or be prevented if treatment by the offending drug is discontinued. Recent research indicates that peroxynitrite (ONOO−) can be a potential indicator to diagnose DILI at an early stage. Therefore, the establishment of an assay to detect and track ONOO− in DILI cases is urgently needed. Here, a FRET-based ratiometric nano fluorescent probe CD–N-I was developed to detect ONOO− with high selectivity and excellent sensitivity. This probe consists of carbon dots and a naphthalimide–isatin peroxynitrite sensing system assembled based on electrostatic interactions. Using CD–N-I we were able to detect exogenous ONOO− in live cells and endogenous ONOO− in APAP-induced liver injury of HepG2 cells.

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Selective FRET nano probe based on carbon dots and naphthalimide–isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

Abstract

Bibliographical note

Funding

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