Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Yueci Wu, Hai Hao Han, Liu He, Li Li, Yi Zang, Jia Li, Xiao Peng He, Yaping Ding, Weiguo Cao, Tony D. James

Research output: Contribution to journalArticlepeer-review

7 Citations (SciVal)

Abstract

Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO in HepG2 cells and endogenous ONOO induced by LPS in RAW 267.4 cells was demonstrated.

Original languageEnglish
Pages (from-to)5051-5054
Number of pages4
JournalChemical Communications
Volume59
Issue number34
Early online date6 Apr 2023
DOIs
Publication statusPublished - 6 Apr 2023

Bibliographical note

Y. W wishes to thank China Scholarship Council, the University of Bath and Shanghai University. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). X.-P. H. thanks the National Natural Science Foundation of China (No. 21788102, 91853201 and 9185920077). J. L. and Y. Z. thank the National Natural Science Foundation of China (No. 82130099, 82151219, 31871414 and 81971265) and the Shanghai Municipal Science and Technology Major Project (No. 22ZR1415200). H.-H. H. thanks the National Natural Science Foundation of China (No. 22107029) and Project funded by the China Postdoctoral Science Foundation (No. 2020M681196).

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • General Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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