Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Yueci Wu, Hai Hao Han, Liu He, Li Li, Yi Zang, Jia Li, Xiao Peng He, Yaping Ding, Weiguo Cao, Tony D. James

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO in HepG2 cells and endogenous ONOO induced by LPS in RAW 267.4 cells was demonstrated.

Original languageEnglish
Pages (from-to)5051-5054
JournalChemical Communications
Volume59
Issue number34
Early online date6 Apr 2023
DOIs
Publication statusPublished - 30 Apr 2023

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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