Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Yueci Wu; Hai Hao Han; Liu He; Li Li; Yi Zang; Jia Li; Xiao Peng He; Yaping Ding; Weiguo Cao; Tony D. James

doi:10.1039/d2cc06425a

Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Yueci Wu, Hai Hao Han, Liu He, Li Li, Yi Zang, Jia Li, Xiao Peng He, Yaping Ding, Weiguo Cao, Tony D. James

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

11 Citations (SciVal)

Abstract

Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO⁻ rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO⁻ in HepG2 cells and endogenous ONOO⁻ induced by LPS in RAW 267.4 cells was demonstrated.

Original language	English
Pages (from-to)	5051-5054
Number of pages	4
Journal	Chemical Communications
Volume	59
Issue number	34
Early online date	6 Apr 2023
DOIs	https://doi.org/10.1039/d2cc06425a
Publication status	Published - 6 Apr 2023

Bibliographical note

Y. W wishes to thank China Scholarship Council, the University of Bath and Shanghai University. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). X.-P. H. thanks the National Natural Science Foundation of China (No. 21788102, 91853201 and 9185920077). J. L. and Y. Z. thank the National Natural Science Foundation of China (No. 82130099, 82151219, 31871414 and 81971265) and the Shanghai Municipal Science and Technology Major Project (No. 22ZR1415200). H.-H. H. thanks the National Natural Science Foundation of China (No. 22107029) and Project funded by the China Postdoctoral Science Foundation (No. 2020M681196).

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO− rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO− in HepG2 cells and endogenous ONOO− induced by LPS in RAW 267.4 cells was demonstrated.",

author = "Yueci Wu and Han, {Hai Hao} and Liu He and Li Li and Yi Zang and Jia Li and He, {Xiao Peng} and Yaping Ding and Weiguo Cao and James, {Tony D.}",

note = "Y. W wishes to thank China Scholarship Council, the University of Bath and Shanghai University. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). X.-P. H. thanks the National Natural Science Foundation of China (No. 21788102, 91853201 and 9185920077). J. L. and Y. Z. thank the National Natural Science Foundation of China (No. 82130099, 82151219, 31871414 and 81971265) and the Shanghai Municipal Science and Technology Major Project (No. 22ZR1415200). H.-H. H. thanks the National Natural Science Foundation of China (No. 22107029) and Project funded by the China Postdoctoral Science Foundation (No. 2020M681196).",

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AU - Wu, Yueci

AU - Han, Hai Hao

AU - He, Liu

AU - Li, Li

AU - Zang, Yi

AU - Li, Jia

AU - He, Xiao Peng

AU - Ding, Yaping

AU - Cao, Weiguo

AU - James, Tony D.

N1 - Y. W wishes to thank China Scholarship Council, the University of Bath and Shanghai University. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). X.-P. H. thanks the National Natural Science Foundation of China (No. 21788102, 91853201 and 9185920077). J. L. and Y. Z. thank the National Natural Science Foundation of China (No. 82130099, 82151219, 31871414 and 81971265) and the Shanghai Municipal Science and Technology Major Project (No. 22ZR1415200). H.-H. H. thanks the National Natural Science Foundation of China (No. 22107029) and Project funded by the China Postdoctoral Science Foundation (No. 2020M681196).

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AB - Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO− rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO− in HepG2 cells and endogenous ONOO− induced by LPS in RAW 267.4 cells was demonstrated.

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Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Abstract

Bibliographical note

ASJC Scopus subject areas

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