TY - UNPB
T1 - Selection of Optimised Ligands by Fluorescence-Activated Bead Sorting
AU - Paul, A.R.
AU - Falsaperna, M.
AU - Lavender, H.
AU - Garrett, M.D.
AU - Serpell, C.J.
PY - 2023/1/10
Y1 - 2023/1/10
N2 - The chemistry of aptamers is largely limited to natural nucleotides, and although modifications of nucleic acids can enhance target aptamer affinity, there has not been a technology for selecting the right modifications in the right locations because enzymatic amplification does not transmit sequence-specific modification information. Here we show the first method for the selection of specific nucleoside modifications that increase aptamer binding efficacy, using the oncoprotein EGFR as a model target. Using fluorescent-activated bead sorting (FABS), we have successfully selected optimized aptamers from a library of >65,000 variations. Hits were identified by tandem mass spectrometry and validated by using an EGFR binding assay and computational docking studies. Our results provide proof of concept for this novel strategy for the selection of chemically optimised aptamers and offer a new method for rapidly synthesising and screening large aptamer libraries to accelerate diagnostic and drug discovery.
AB - The chemistry of aptamers is largely limited to natural nucleotides, and although modifications of nucleic acids can enhance target aptamer affinity, there has not been a technology for selecting the right modifications in the right locations because enzymatic amplification does not transmit sequence-specific modification information. Here we show the first method for the selection of specific nucleoside modifications that increase aptamer binding efficacy, using the oncoprotein EGFR as a model target. Using fluorescent-activated bead sorting (FABS), we have successfully selected optimized aptamers from a library of >65,000 variations. Hits were identified by tandem mass spectrometry and validated by using an EGFR binding assay and computational docking studies. Our results provide proof of concept for this novel strategy for the selection of chemically optimised aptamers and offer a new method for rapidly synthesising and screening large aptamer libraries to accelerate diagnostic and drug discovery.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-85148933025&partnerID=MN8TOARS
U2 - 10.26434/chemrxiv-2023-d7ss7
DO - 10.26434/chemrxiv-2023-d7ss7
M3 - Working paper
BT - Selection of Optimised Ligands by Fluorescence-Activated Bead Sorting
PB - ChemRxiv
ER -