SecDF of Bacillus subtilis, a molecular siamese twin required for the efficient secretion of proteins

Albert Bolhuis, Cees P. Broekhuizen, Alexei Sorokin, Maarten L. Van Roosmalen, Gerard Venema, Sierd Bron, Wim J. Quax, Jan Maarten Van Dijl

Research output: Contribution to journalArticle

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Abstract

In the present studies, we show that the SecD and SecF equivalents of the Gram-positive bacterium Bacillus subtilis are jointly present in one polypeptide, denoted SecDF, that is required to maintain a high capacity for protein secretion. Unlike the SecD subunit of the pre-protein translocase of Escherichia coli, SecDF of B. subtilis was not required for the release of a mature secretory protein from the membrane, indicating that SecDF is involved in earlier translocation steps. Strains lacking intact SecDF showed a cold- sensitive phenotype, which was exacerbated by high level production of secretory proteins, indicating that protein translocation in B. subtilis is intrinsically cold-sensitive. Comparison with SecD and SecF proteins from other organisms revealed the presence of 10 conserved regions in SecDF, some of which appear to be important for SecDF function. Interestingly, the SecDF protein of B. subtilis has 12 putative transmembrane domains. Thus, SecDF does not only show sequence similarity but also structural similarity to secondary solute transporters. Our data suggest that SecDF of B. subtilis represents a novel type of the SecD and SecF proteins, which seems to be present in at least two other organisms.

Original languageEnglish
Pages (from-to)21217-21224
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number33
DOIs
Publication statusPublished - 14 Aug 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bolhuis, A., Broekhuizen, C. P., Sorokin, A., Van Roosmalen, M. L., Venema, G., Bron, S., ... Van Dijl, J. M. (1998). SecDF of Bacillus subtilis, a molecular siamese twin required for the efficient secretion of proteins. Journal of Biological Chemistry, 273(33), 21217-21224. https://doi.org/10.1074/jbc.273.33.21217

SecDF of Bacillus subtilis, a molecular siamese twin required for the efficient secretion of proteins. / Bolhuis, Albert; Broekhuizen, Cees P.; Sorokin, Alexei; Van Roosmalen, Maarten L.; Venema, Gerard; Bron, Sierd; Quax, Wim J.; Van Dijl, Jan Maarten.

In: Journal of Biological Chemistry, Vol. 273, No. 33, 14.08.1998, p. 21217-21224.

Research output: Contribution to journalArticle

Bolhuis, A, Broekhuizen, CP, Sorokin, A, Van Roosmalen, ML, Venema, G, Bron, S, Quax, WJ & Van Dijl, JM 1998, 'SecDF of Bacillus subtilis, a molecular siamese twin required for the efficient secretion of proteins', Journal of Biological Chemistry, vol. 273, no. 33, pp. 21217-21224. https://doi.org/10.1074/jbc.273.33.21217
Bolhuis, Albert ; Broekhuizen, Cees P. ; Sorokin, Alexei ; Van Roosmalen, Maarten L. ; Venema, Gerard ; Bron, Sierd ; Quax, Wim J. ; Van Dijl, Jan Maarten. / SecDF of Bacillus subtilis, a molecular siamese twin required for the efficient secretion of proteins. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 33. pp. 21217-21224.
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