S cysteine-rich (SCR) binding domain analysis of the Brassica self-incompatibility S-locus receptor kinase

B P Kemp, J Doughty

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

@Brassica self-incompatibility, a highly discriminating outbreeding mechanism, has become a paradigm for the study of plant cell-cell communications. When self-pollen lands on a stigma, the male ligand S cysteine-rich (SCR), which is present in the pollen coat, is transmitted to the female receptor, S-locus receptor kinase (SRK). SRK is a membrane-spanning serine/threonine receptor kinase present in the stigmatic papillar cell membrane. Haplotype-specific binding of SCR to SRK brings about pollen rejection. The extracellular receptor domain of SRK (eSRK) is responsible for binding SCR. Based on sequence homology, eSRK can be divided into three subdomains: B lectin-like, hypervariable, and PAN. Biochemical analysis of these subdomains showed that the hypervariable subdomain is responsible for most of the SCR binding capacity of eSRK, whereas the B lectin-like and PAN domains have little, if any, affinity for SCR. Fine mapping of the SCR binding region of SRK using a peptide array revealed a region of the hypervariable subdomain that plays a key role in binding the SCR molecule. We show that residues within the hypervariable subdomain define SRK binding and are likely to be involved in defining haplotype specificity.
Original languageEnglish
Pages (from-to)619-629
Number of pages11
JournalNew Phytologist
Volume175
Issue number4
DOIs
Publication statusPublished - 2007

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Brassica
Cysteine
cysteine
phosphotransferases (kinases)
receptors
loci
Pollen
Haplotypes
pollen
lectins
haplotypes
Protein-Serine-Threonine Kinases
Plant Cells
Sequence Homology
S-receptor kinase
outbreeding
cell communication
Cell Communication
binding capacity
sequence homology

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S cysteine-rich (SCR) binding domain analysis of the Brassica self-incompatibility S-locus receptor kinase. / Kemp, B P; Doughty, J.

In: New Phytologist, Vol. 175, No. 4, 2007, p. 619-629.

Research output: Contribution to journalArticle

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