Ruminant and chicken: Important sources of campylobacteriosis in France despite a variation of source attribution in 2009 and 2015

Amandine Thépault, Valérie Rose, Ségolène Quesne, Typhaine Poezevara, Véronique Béven, Edouard Hirchaud, Fabrice Touzain, Pierrick Lucas, Guillaume Méric, Leonardos Mageiros, Samuel K. Sheppard, Marianne Chemaly, Katell Rivoal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pathogen source attribution studies are a useful tool for identifying reservoirs of human infection. Based on Multilocus Sequence Typing (MLST) data, such studies have identified chicken as a major source of C. jejuni human infection. The use of whole genome sequence-based typing methods offers potential to improve the precision of attribution beyond that which is possible from 7 MLST loci. Using published data and 156 novel C. jejuni genomes sequenced in this study, we performed probabilistic host source attribution of clinical C. jejuni isolates from France using three types of genotype data: comparative genomic fingerprints; MLST genes; 15 host segregating genes previously identified by whole genome sequencing. Consistent with previous studies, chicken was an important source of campylobacteriosis in France (31-63% of clinical isolates assigned). There was also evidence that ruminants are a source (22-55% of clinical isolates assigned), suggesting that further investigation of potential transmission routes from ruminants to human would be useful. Additionally, we found evidence of environmental and pet sources. However, the relative importance as sources varied according to the year of isolation and the genotyping technique used. Annual variations in attribution emphasize the dynamic nature of zoonotic transmission and the need to perform source attribution regularly.

Original languageEnglish
Article number9305
Pages (from-to)1-10
Number of pages10
JournalScientific Reports
Volume8
Issue number1
Early online date18 Jun 2018
DOIs
Publication statusPublished - 18 Jun 2018

Fingerprint

Multilocus Sequence Typing
Ruminants
France
Chickens
Genome
Genotyping Techniques
Pets
Zoonoses
Dermatoglyphics
Infection
Genes
Genotype

ASJC Scopus subject areas

  • General

Cite this

Ruminant and chicken : Important sources of campylobacteriosis in France despite a variation of source attribution in 2009 and 2015. / Thépault, Amandine; Rose, Valérie; Quesne, Ségolène; Poezevara, Typhaine; Béven, Véronique; Hirchaud, Edouard; Touzain, Fabrice; Lucas, Pierrick; Méric, Guillaume; Mageiros, Leonardos; Sheppard, Samuel K.; Chemaly, Marianne; Rivoal, Katell.

In: Scientific Reports, Vol. 8, No. 1, 9305, 18.06.2018, p. 1-10.

Research output: Contribution to journalArticle

Thépault, A, Rose, V, Quesne, S, Poezevara, T, Béven, V, Hirchaud, E, Touzain, F, Lucas, P, Méric, G, Mageiros, L, Sheppard, SK, Chemaly, M & Rivoal, K 2018, 'Ruminant and chicken: Important sources of campylobacteriosis in France despite a variation of source attribution in 2009 and 2015', Scientific Reports, vol. 8, no. 1, 9305, pp. 1-10. https://doi.org/10.1038/s41598-018-27558-z
Thépault, Amandine ; Rose, Valérie ; Quesne, Ségolène ; Poezevara, Typhaine ; Béven, Véronique ; Hirchaud, Edouard ; Touzain, Fabrice ; Lucas, Pierrick ; Méric, Guillaume ; Mageiros, Leonardos ; Sheppard, Samuel K. ; Chemaly, Marianne ; Rivoal, Katell. / Ruminant and chicken : Important sources of campylobacteriosis in France despite a variation of source attribution in 2009 and 2015. In: Scientific Reports. 2018 ; Vol. 8, No. 1. pp. 1-10.
@article{9f8a3717a77146fdaf6a2725d0d08e9e,
title = "Ruminant and chicken: Important sources of campylobacteriosis in France despite a variation of source attribution in 2009 and 2015",
abstract = "Pathogen source attribution studies are a useful tool for identifying reservoirs of human infection. Based on Multilocus Sequence Typing (MLST) data, such studies have identified chicken as a major source of C. jejuni human infection. The use of whole genome sequence-based typing methods offers potential to improve the precision of attribution beyond that which is possible from 7 MLST loci. Using published data and 156 novel C. jejuni genomes sequenced in this study, we performed probabilistic host source attribution of clinical C. jejuni isolates from France using three types of genotype data: comparative genomic fingerprints; MLST genes; 15 host segregating genes previously identified by whole genome sequencing. Consistent with previous studies, chicken was an important source of campylobacteriosis in France (31-63{\%} of clinical isolates assigned). There was also evidence that ruminants are a source (22-55{\%} of clinical isolates assigned), suggesting that further investigation of potential transmission routes from ruminants to human would be useful. Additionally, we found evidence of environmental and pet sources. However, the relative importance as sources varied according to the year of isolation and the genotyping technique used. Annual variations in attribution emphasize the dynamic nature of zoonotic transmission and the need to perform source attribution regularly.",
author = "Amandine Th{\'e}pault and Val{\'e}rie Rose and S{\'e}gol{\`e}ne Quesne and Typhaine Poezevara and V{\'e}ronique B{\'e}ven and Edouard Hirchaud and Fabrice Touzain and Pierrick Lucas and Guillaume M{\'e}ric and Leonardos Mageiros and Sheppard, {Samuel K.} and Marianne Chemaly and Katell Rivoal",
year = "2018",
month = "6",
day = "18",
doi = "10.1038/s41598-018-27558-z",
language = "English",
volume = "8",
pages = "1--10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Research",
number = "1",

}

TY - JOUR

T1 - Ruminant and chicken

T2 - Important sources of campylobacteriosis in France despite a variation of source attribution in 2009 and 2015

AU - Thépault, Amandine

AU - Rose, Valérie

AU - Quesne, Ségolène

AU - Poezevara, Typhaine

AU - Béven, Véronique

AU - Hirchaud, Edouard

AU - Touzain, Fabrice

AU - Lucas, Pierrick

AU - Méric, Guillaume

AU - Mageiros, Leonardos

AU - Sheppard, Samuel K.

AU - Chemaly, Marianne

AU - Rivoal, Katell

PY - 2018/6/18

Y1 - 2018/6/18

N2 - Pathogen source attribution studies are a useful tool for identifying reservoirs of human infection. Based on Multilocus Sequence Typing (MLST) data, such studies have identified chicken as a major source of C. jejuni human infection. The use of whole genome sequence-based typing methods offers potential to improve the precision of attribution beyond that which is possible from 7 MLST loci. Using published data and 156 novel C. jejuni genomes sequenced in this study, we performed probabilistic host source attribution of clinical C. jejuni isolates from France using three types of genotype data: comparative genomic fingerprints; MLST genes; 15 host segregating genes previously identified by whole genome sequencing. Consistent with previous studies, chicken was an important source of campylobacteriosis in France (31-63% of clinical isolates assigned). There was also evidence that ruminants are a source (22-55% of clinical isolates assigned), suggesting that further investigation of potential transmission routes from ruminants to human would be useful. Additionally, we found evidence of environmental and pet sources. However, the relative importance as sources varied according to the year of isolation and the genotyping technique used. Annual variations in attribution emphasize the dynamic nature of zoonotic transmission and the need to perform source attribution regularly.

AB - Pathogen source attribution studies are a useful tool for identifying reservoirs of human infection. Based on Multilocus Sequence Typing (MLST) data, such studies have identified chicken as a major source of C. jejuni human infection. The use of whole genome sequence-based typing methods offers potential to improve the precision of attribution beyond that which is possible from 7 MLST loci. Using published data and 156 novel C. jejuni genomes sequenced in this study, we performed probabilistic host source attribution of clinical C. jejuni isolates from France using three types of genotype data: comparative genomic fingerprints; MLST genes; 15 host segregating genes previously identified by whole genome sequencing. Consistent with previous studies, chicken was an important source of campylobacteriosis in France (31-63% of clinical isolates assigned). There was also evidence that ruminants are a source (22-55% of clinical isolates assigned), suggesting that further investigation of potential transmission routes from ruminants to human would be useful. Additionally, we found evidence of environmental and pet sources. However, the relative importance as sources varied according to the year of isolation and the genotyping technique used. Annual variations in attribution emphasize the dynamic nature of zoonotic transmission and the need to perform source attribution regularly.

UR - http://www.scopus.com/inward/record.url?scp=85048801479&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-27558-z

DO - 10.1038/s41598-018-27558-z

M3 - Article

C2 - 29915208

AN - SCOPUS:85048801479

VL - 8

SP - 1

EP - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 9305

ER -