Role of Candida albicans Tem1 in mitotic exit and cytokinesis

Stephen W. Milne, Jill Cheetham, Deborah Lloyd, Sophie Shaw, Karen Moore, Konrad H. Paszkiewicz, Stephen J. Aves, Steven Bates

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Candida albicans demonstrates three main growth morphologies: yeast, pseudohyphal and true hyphal forms. Cell separation is distinct in these morphological forms and the process of separation is closely linked to the completion of mitosis and cytokinesis. In Saccharomyces cerevisiae the small GTPase Tem1 is known to initiate the mitotic exit network, a signalling pathway involved in signalling the end of mitosis and initiating cytokinesis and cell separation. Here we have characterised the role of Tem1 in C. albicans, and demonstrate that it is essential for mitotic exit and cytokinesis, and that this essential function is signalled through the kinase Cdc15. Cells depleted of Tem1 displayed highly polarised growth but ultimately failed to both complete cytokinesis and re-enter the cell cycle following nuclear division. Consistent with its role in activating the mitotic exit network Tem1 localises to spindle pole bodies in a cell cycle-dependent manner. Ultimately, the mitotic exit network in C. albicans appears to co-ordinate the sequential processes of mitotic exit, cytokinesis and cell separation.

Original languageEnglish
Pages (from-to)84-95
Number of pages12
JournalFungal Genetics and Biology
Volume69
DOIs
Publication statusPublished - 2014

Fingerprint

Cytokinesis
Candida albicans
Cell Separation
Mitosis
Cell Cycle
Spindle Pole Bodies
Cell Nucleus Division
Monomeric GTP-Binding Proteins
Growth
Saccharomyces cerevisiae
Phosphotransferases
Yeasts

Keywords

  • Candida albicans
  • Cell cycle
  • Cell separation
  • Cytokinesis
  • Mitotic exit
  • Tem1

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Cite this

Milne, S. W., Cheetham, J., Lloyd, D., Shaw, S., Moore, K., Paszkiewicz, K. H., ... Bates, S. (2014). Role of Candida albicans Tem1 in mitotic exit and cytokinesis. Fungal Genetics and Biology, 69, 84-95. https://doi.org/10.1016/j.fgb.2014.06.007

Role of Candida albicans Tem1 in mitotic exit and cytokinesis. / Milne, Stephen W.; Cheetham, Jill; Lloyd, Deborah; Shaw, Sophie; Moore, Karen; Paszkiewicz, Konrad H.; Aves, Stephen J.; Bates, Steven.

In: Fungal Genetics and Biology, Vol. 69, 2014, p. 84-95.

Research output: Contribution to journalArticle

Milne, SW, Cheetham, J, Lloyd, D, Shaw, S, Moore, K, Paszkiewicz, KH, Aves, SJ & Bates, S 2014, 'Role of Candida albicans Tem1 in mitotic exit and cytokinesis', Fungal Genetics and Biology, vol. 69, pp. 84-95. https://doi.org/10.1016/j.fgb.2014.06.007
Milne, Stephen W. ; Cheetham, Jill ; Lloyd, Deborah ; Shaw, Sophie ; Moore, Karen ; Paszkiewicz, Konrad H. ; Aves, Stephen J. ; Bates, Steven. / Role of Candida albicans Tem1 in mitotic exit and cytokinesis. In: Fungal Genetics and Biology. 2014 ; Vol. 69. pp. 84-95.
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