Role of extracellular chymoelastase in the virulence of for

R J St Leger, P K Durrands, A Keith Charnley, Richard M Cooper

Research output: Contribution to journalArticle

Abstract

The relationship between extracellular chymoelastase (Pr1) and the virulence of Metarhizium anisopliae for Manduca sexta was studied using either IgG antibodies vs Pr1 or turkey egg white inhibitor, a potent specific inhibitor of Pr1. Treatment of M. sexta with the inhibitor during infection significantly delayed mortality. The inhibitor also reduced melanization of cuticle (an early host response to infection) and invasion of the hemolymph, as well as maintained the host's growth rate. The incorporation of antibodies or inhibitor delayed penetration of cuticle, but did not affect spore viability or prevent growth and formation of appressoria on the cuticle surface. This suggests that inhibition of Pr1 reduced infection by limiting fungal penetration of the insect cuticle. In vitro studies using inhibitor showed that the accumulation of protein degradation products from cuticle, including ammonia, was dependent on active Pr1. This confirms the major role of Pr1 in solubilizing cuticle proteins and making them available for nutrition. It is concluded that Pr1 serves as a virulence factor by causing the localized destruction of cuticle proteins, which enables rapid invasion of the host with concomitant provision of nutrients.
Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalJournal of Invertebrate Pathology
Volume52
Issue number2
DOIs
Publication statusPublished - 1988

Fingerprint

cuticle
virulence
Manduca sexta
inhibitor
turkey eggs
infection
insect cuticle
melanization
antibodies
appressoria
Metarhizium anisopliae
egg albumen
protein degradation
solubilization
in vitro studies
hemolymph
ammonia
proteins
antibody
protein

Cite this

St Leger, R. J., Durrands, P. K., Charnley, A. K., & Cooper, R. M. (1988). Role of extracellular chymoelastase in the virulence of for. Journal of Invertebrate Pathology, 52(2), 285-293. https://doi.org/10.1016/0022-2011(88)90137-1

Role of extracellular chymoelastase in the virulence of for. / St Leger, R J; Durrands, P K; Charnley, A Keith; Cooper, Richard M.

In: Journal of Invertebrate Pathology, Vol. 52, No. 2, 1988, p. 285-293.

Research output: Contribution to journalArticle

St Leger, RJ, Durrands, PK, Charnley, AK & Cooper, RM 1988, 'Role of extracellular chymoelastase in the virulence of for', Journal of Invertebrate Pathology, vol. 52, no. 2, pp. 285-293. https://doi.org/10.1016/0022-2011(88)90137-1
St Leger, R J ; Durrands, P K ; Charnley, A Keith ; Cooper, Richard M. / Role of extracellular chymoelastase in the virulence of for. In: Journal of Invertebrate Pathology. 1988 ; Vol. 52, No. 2. pp. 285-293.
@article{b105dab7107a42c8be6504ff06bef734,
title = "Role of extracellular chymoelastase in the virulence of for",
abstract = "The relationship between extracellular chymoelastase (Pr1) and the virulence of Metarhizium anisopliae for Manduca sexta was studied using either IgG antibodies vs Pr1 or turkey egg white inhibitor, a potent specific inhibitor of Pr1. Treatment of M. sexta with the inhibitor during infection significantly delayed mortality. The inhibitor also reduced melanization of cuticle (an early host response to infection) and invasion of the hemolymph, as well as maintained the host's growth rate. The incorporation of antibodies or inhibitor delayed penetration of cuticle, but did not affect spore viability or prevent growth and formation of appressoria on the cuticle surface. This suggests that inhibition of Pr1 reduced infection by limiting fungal penetration of the insect cuticle. In vitro studies using inhibitor showed that the accumulation of protein degradation products from cuticle, including ammonia, was dependent on active Pr1. This confirms the major role of Pr1 in solubilizing cuticle proteins and making them available for nutrition. It is concluded that Pr1 serves as a virulence factor by causing the localized destruction of cuticle proteins, which enables rapid invasion of the host with concomitant provision of nutrients.",
author = "{St Leger}, {R J} and Durrands, {P K} and Charnley, {A Keith} and Cooper, {Richard M}",
year = "1988",
doi = "10.1016/0022-2011(88)90137-1",
language = "English",
volume = "52",
pages = "285--293",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "Elsevier Academic Press Inc",
number = "2",

}

TY - JOUR

T1 - Role of extracellular chymoelastase in the virulence of for

AU - St Leger, R J

AU - Durrands, P K

AU - Charnley, A Keith

AU - Cooper, Richard M

PY - 1988

Y1 - 1988

N2 - The relationship between extracellular chymoelastase (Pr1) and the virulence of Metarhizium anisopliae for Manduca sexta was studied using either IgG antibodies vs Pr1 or turkey egg white inhibitor, a potent specific inhibitor of Pr1. Treatment of M. sexta with the inhibitor during infection significantly delayed mortality. The inhibitor also reduced melanization of cuticle (an early host response to infection) and invasion of the hemolymph, as well as maintained the host's growth rate. The incorporation of antibodies or inhibitor delayed penetration of cuticle, but did not affect spore viability or prevent growth and formation of appressoria on the cuticle surface. This suggests that inhibition of Pr1 reduced infection by limiting fungal penetration of the insect cuticle. In vitro studies using inhibitor showed that the accumulation of protein degradation products from cuticle, including ammonia, was dependent on active Pr1. This confirms the major role of Pr1 in solubilizing cuticle proteins and making them available for nutrition. It is concluded that Pr1 serves as a virulence factor by causing the localized destruction of cuticle proteins, which enables rapid invasion of the host with concomitant provision of nutrients.

AB - The relationship between extracellular chymoelastase (Pr1) and the virulence of Metarhizium anisopliae for Manduca sexta was studied using either IgG antibodies vs Pr1 or turkey egg white inhibitor, a potent specific inhibitor of Pr1. Treatment of M. sexta with the inhibitor during infection significantly delayed mortality. The inhibitor also reduced melanization of cuticle (an early host response to infection) and invasion of the hemolymph, as well as maintained the host's growth rate. The incorporation of antibodies or inhibitor delayed penetration of cuticle, but did not affect spore viability or prevent growth and formation of appressoria on the cuticle surface. This suggests that inhibition of Pr1 reduced infection by limiting fungal penetration of the insect cuticle. In vitro studies using inhibitor showed that the accumulation of protein degradation products from cuticle, including ammonia, was dependent on active Pr1. This confirms the major role of Pr1 in solubilizing cuticle proteins and making them available for nutrition. It is concluded that Pr1 serves as a virulence factor by causing the localized destruction of cuticle proteins, which enables rapid invasion of the host with concomitant provision of nutrients.

UR - http://dx.doi.org/10.1016/0022-2011(88)90137-1

U2 - 10.1016/0022-2011(88)90137-1

DO - 10.1016/0022-2011(88)90137-1

M3 - Article

VL - 52

SP - 285

EP - 293

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

IS - 2

ER -