RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

O Terenius; A Papanicolaou; Jennifer S Garbutt; I Eleftherianos; H Huvenne; S Kanginakudru; M Albrechtsen; C J An; J L Aymeric; A Barthel; P Bebas; K Bitra; A Bravo; F Chevalieri; D P Collinge; C M Crava; R A de Maagd; B Duvic; M Erlandson; I Faye; G Felfoldi; H Fujiwara; R Futahashi; A S Gandhe; H S Gatehouse; L N Gatehouse; J M Giebultowicz; I Gomez; C J P Grimmelikhuijzen; A T Groot; F Hauser; D G Heckel; D D Hegedus; S Hrycaj; L H Huang; J J Hull; K Iatrou; M Iga; M R Kanost; J Kotwica; C Y Li; J H Li; J S Liu; M Lundmark; S Matsumoto; M Meyering-Vos; Peter J Millichap; A Monteiro; N Mrinal; T Niimi; D Nowara; A Ohnishi; V Oostra; K Ozaki; M Papakonstantinou; A Popadic; M V Rajam; S Saenko; R M Simpson; M Soberon; M R Strand; S Tomita; U Toprak; P Wang; C W Wee; S Whyard; W Q Zhang; J Nagaraju; R H Ffrench-Constant; S Herrero; K Gordon; L Swelters; G Smagghe

doi:10.1016/j.jinsphys.2010.11.006

RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

O Terenius, A Papanicolaou, Jennifer S Garbutt, I Eleftherianos, H Huvenne, S Kanginakudru, M Albrechtsen, C J An, J L Aymeric, A Barthel, P Bebas, K Bitra, A Bravo, F Chevalieri, D P Collinge, C M Crava, R A de Maagd, B Duvic, M Erlandson, I FayeG Felfoldi, H Fujiwara, R Futahashi, A S Gandhe, H S Gatehouse, L N Gatehouse, J M Giebultowicz, I Gomez, C J P Grimmelikhuijzen, A T Groot, F Hauser, D G Heckel, D D Hegedus, S Hrycaj, L H Huang, J J Hull, K Iatrou, M Iga, M R Kanost, J Kotwica, C Y Li, J H Li, J S Liu, M Lundmark, S Matsumoto, M Meyering-Vos, Peter J Millichap, A Monteiro, N Mrinal, T Niimi, D Nowara, A Ohnishi, V Oostra, K Ozaki, M Papakonstantinou, A Popadic, M V Rajam, S Saenko, R M Simpson, M Soberon, M R Strand, S Tomita, U Toprak, P Wang, C W Wee, S Whyard, W Q Zhang, J Nagaraju, R H Ffrench-Constant, S Herrero, K Gordon, L Swelters, G Smagghe

Department of Life Sciences

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Abstract

Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	231-245
Number of pages	15
Journal	Journal of Insect Physiology
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/j.jinsphys.2010.11.006
Publication status	Published - Feb 2011

Keywords

gene function
delivery methods
tissue uptake
dsRNA properties
Lepidoptera
RNA interference

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10.1016/j.jinsphys.2010.11.006

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Terenius, O., Papanicolaou, A., Garbutt, J. S., Eleftherianos, I., Huvenne, H., Kanginakudru, S., Albrechtsen, M., An, C. J., Aymeric, J. L., Barthel, A., Bebas, P., Bitra, K., Bravo, A., Chevalieri, F., Collinge, D. P., Crava, C. M., de Maagd, R. A., Duvic, B., Erlandson, M., ... Smagghe, G. (2011). RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design. Journal of Insect Physiology, 57(2), 231-245. https://doi.org/10.1016/j.jinsphys.2010.11.006

Terenius, O, Papanicolaou, A, Garbutt, JS, Eleftherianos, I, Huvenne, H, Kanginakudru, S, Albrechtsen, M, An, CJ, Aymeric, JL, Barthel, A, Bebas, P, Bitra, K, Bravo, A, Chevalieri, F, Collinge, DP, Crava, CM, de Maagd, RA, Duvic, B, Erlandson, M, Faye, I, Felfoldi, G, Fujiwara, H, Futahashi, R, Gandhe, AS, Gatehouse, HS, Gatehouse, LN, Giebultowicz, JM, Gomez, I, Grimmelikhuijzen, CJP, Groot, AT, Hauser, F, Heckel, DG, Hegedus, DD, Hrycaj, S, Huang, LH, Hull, JJ, Iatrou, K, Iga, M, Kanost, MR, Kotwica, J, Li, CY, Li, JH, Liu, JS, Lundmark, M, Matsumoto, S, Meyering-Vos, M, Millichap, PJ, Monteiro, A, Mrinal, N, Niimi, T, Nowara, D, Ohnishi, A, Oostra, V, Ozaki, K, Papakonstantinou, M, Popadic, A, Rajam, MV, Saenko, S, Simpson, RM, Soberon, M, Strand, MR, Tomita, S, Toprak, U, Wang, P, Wee, CW, Whyard, S, Zhang, WQ, Nagaraju, J, Ffrench-Constant, RH, Herrero, S, Gordon, K, Swelters, L & Smagghe, G 2011, 'RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design', Journal of Insect Physiology, vol. 57, no. 2, pp. 231-245. https://doi.org/10.1016/j.jinsphys.2010.11.006

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title = "RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design",

abstract = "Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.",

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author = "O Terenius and A Papanicolaou and Garbutt, {Jennifer S} and I Eleftherianos and H Huvenne and S Kanginakudru and M Albrechtsen and An, {C J} and Aymeric, {J L} and A Barthel and P Bebas and K Bitra and A Bravo and F Chevalieri and Collinge, {D P} and Crava, {C M} and {de Maagd}, {R A} and B Duvic and M Erlandson and I Faye and G Felfoldi and H Fujiwara and R Futahashi and Gandhe, {A S} and Gatehouse, {H S} and Gatehouse, {L N} and Giebultowicz, {J M} and I Gomez and Grimmelikhuijzen, {C J P} and Groot, {A T} and F Hauser and Heckel, {D G} and Hegedus, {D D} and S Hrycaj and Huang, {L H} and Hull, {J J} and K Iatrou and M Iga and Kanost, {M R} and J Kotwica and Li, {C Y} and Li, {J H} and Liu, {J S} and M Lundmark and S Matsumoto and M Meyering-Vos and Millichap, {Peter J} and A Monteiro and N Mrinal and T Niimi and D Nowara and A Ohnishi and V Oostra and K Ozaki and M Papakonstantinou and A Popadic and Rajam, {M V} and S Saenko and Simpson, {R M} and M Soberon and Strand, {M R} and S Tomita and U Toprak and P Wang and Wee, {C W} and S Whyard and Zhang, {W Q} and J Nagaraju and Ffrench-Constant, {R H} and S Herrero and K Gordon and L Swelters and G Smagghe",

year = "2011",

month = feb,

doi = "10.1016/j.jinsphys.2010.11.006",

language = "English",

volume = "57",

pages = "231--245",

journal = "Journal of Insect Physiology",

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T1 - RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

AU - Terenius, O

AU - Papanicolaou, A

AU - Garbutt, Jennifer S

AU - Eleftherianos, I

AU - Huvenne, H

AU - Kanginakudru, S

AU - Albrechtsen, M

AU - An, C J

AU - Aymeric, J L

AU - Barthel, A

AU - Bebas, P

AU - Bitra, K

AU - Bravo, A

AU - Chevalieri, F

AU - Collinge, D P

AU - Crava, C M

AU - de Maagd, R A

AU - Duvic, B

AU - Erlandson, M

AU - Faye, I

AU - Felfoldi, G

AU - Fujiwara, H

AU - Futahashi, R

AU - Gandhe, A S

AU - Gatehouse, H S

AU - Gatehouse, L N

AU - Giebultowicz, J M

AU - Gomez, I

AU - Grimmelikhuijzen, C J P

AU - Groot, A T

AU - Hauser, F

AU - Heckel, D G

AU - Hegedus, D D

AU - Hrycaj, S

AU - Huang, L H

AU - Hull, J J

AU - Iatrou, K

AU - Iga, M

AU - Kanost, M R

AU - Kotwica, J

AU - Li, C Y

AU - Li, J H

AU - Liu, J S

AU - Lundmark, M

AU - Matsumoto, S

AU - Meyering-Vos, M

AU - Millichap, Peter J

AU - Monteiro, A

AU - Mrinal, N

AU - Niimi, T

AU - Nowara, D

AU - Ohnishi, A

AU - Oostra, V

AU - Ozaki, K

AU - Papakonstantinou, M

AU - Popadic, A

AU - Rajam, M V

AU - Saenko, S

AU - Simpson, R M

AU - Soberon, M

AU - Strand, M R

AU - Tomita, S

AU - Toprak, U

AU - Wang, P

AU - Wee, C W

AU - Whyard, S

AU - Zhang, W Q

AU - Nagaraju, J

AU - Ffrench-Constant, R H

AU - Herrero, S

AU - Gordon, K

AU - Swelters, L

AU - Smagghe, G

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N2 - Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.

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KW - gene function

KW - delivery methods

KW - tissue uptake

KW - dsRNA properties

KW - Lepidoptera

KW - RNA interference

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RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

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Keywords

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