Reverse transcriptase-mediated tropism switching in Bordetella bacteriophage

Minghsun Liu; Rajendar Deora; Sergei R Doulatov; Mari Gingery; Frederick A Eiserling; Andrew Preston; Duncan J Maskell; Robert W Simons; Peggy A Cotter; Julian Parkhill; Jeff F Miller

doi:10.1126/science.1067467

Reverse transcriptase-mediated tropism switching in Bordetella bacteriophage

Minghsun Liu, Rajendar Deora, Sergei R Doulatov, Mari Gingery, Frederick A Eiserling, Andrew Preston, Duncan J Maskell, Robert W Simons, Peggy A Cotter, Julian Parkhill, Jeff F Miller

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

203 Citations (SciVal)

Abstract

Host-pathogen interactions are often driven by mechanisms that promote genetic variability. We have identified a group of temperate bacteriophages that generate diversity in a gene, designated mtd (major tropism determinant), which specifies tropism for receptor molecules on host Bordetella species. Tropism switching is the result of a template-dependent, reverse transcriptase-mediated process that introduces nucleotide substitutions at defined locations within mtd. This cassette-based mechanism is capable of providing a vast repertoire of potential ligand-receptor interactions.

Original language	English
Pages (from-to)	2091-2094
Number of pages	4
Journal	Science
Volume	295
Issue number	5562
DOIs	https://doi.org/10.1126/science.1067467
Publication status	Published - 2002

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title = "Reverse transcriptase-mediated tropism switching in Bordetella bacteriophage",

abstract = "Host-pathogen interactions are often driven by mechanisms that promote genetic variability. We have identified a group of temperate bacteriophages that generate diversity in a gene, designated mtd (major tropism determinant), which specifies tropism for receptor molecules on host Bordetella species. Tropism switching is the result of a template-dependent, reverse transcriptase-mediated process that introduces nucleotide substitutions at defined locations within mtd. This cassette-based mechanism is capable of providing a vast repertoire of potential ligand-receptor interactions.",

author = "Minghsun Liu and Rajendar Deora and Doulatov, {Sergei R} and Mari Gingery and Eiserling, {Frederick A} and Andrew Preston and Maskell, {Duncan J} and Simons, {Robert W} and Cotter, {Peggy A} and Julian Parkhill and Miller, {Jeff F}",

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doi = "10.1126/science.1067467",

language = "English",

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AU - Liu, Minghsun

AU - Deora, Rajendar

AU - Doulatov, Sergei R

AU - Gingery, Mari

AU - Eiserling, Frederick A

AU - Preston, Andrew

AU - Maskell, Duncan J

AU - Simons, Robert W

AU - Cotter, Peggy A

AU - Parkhill, Julian

AU - Miller, Jeff F

PY - 2002

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AB - Host-pathogen interactions are often driven by mechanisms that promote genetic variability. We have identified a group of temperate bacteriophages that generate diversity in a gene, designated mtd (major tropism determinant), which specifies tropism for receptor molecules on host Bordetella species. Tropism switching is the result of a template-dependent, reverse transcriptase-mediated process that introduces nucleotide substitutions at defined locations within mtd. This cassette-based mechanism is capable of providing a vast repertoire of potential ligand-receptor interactions.

UR - http://dx.doi.org/10.1126/science.1067467

U2 - 10.1126/science.1067467

DO - 10.1126/science.1067467

M3 - Article

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VL - 295

SP - 2091

EP - 2094

JO - Science

JF - Science

IS - 5562

ER -

Reverse transcriptase-mediated tropism switching in Bordetella bacteriophage

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