Resolving the aphid resistance locus Sd-1 on a BAC contig within a sub-telomeric region of Malus linkage group 7

Volkan Cevik, Graham J King

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aphids cause serious physical and economic damage to most major crops throughout the world, and there is a pressing requirement to isolate genes conferring aphid resistance. The Sd-1 locus in Malus spp. (apple) confers resistance against the rosy leaf-curling aphid (Dysaphis devecta Wlk.), and was recently positioned within a 1.3-cM region on linkage group 7, flanked by molecular markers. These markers were used as a basis for development of a BAC contig spanning the locus, together with adapter-mediated amplification of flanking sequences to obtain BAC insert-end sequences, and fingerprinting of BAC clones. Approximately 800 kb of the Sd-1 genomic region was covered by 19 overlapping BACs, with an average insert size of 75-150 kb. The physical-genetic distance ratio was estimated at 460 kb/cM, although the distribution of recombination events was irregular with respect to estimated physical distance. Recombinant analysis and development of new markers allowed Sd-1 to be positioned within an interval of approximately 180 kb located on either of two overlapping BACs. From one of these, an insert end sequence showed a significant degree of similarity to nucleotide binding site-leucine rich repeat (NBS-LRR) resistance genes. Fluorescent in situ hybridization (FISH) of BAC clones within the contig enabled positioning and orientation of the locus within a euchromatic region, very close to the telomere of linkage group 7.

Original languageEnglish
Pages (from-to)939-945
Number of pages7
JournalGenome Biology
Volume45
Issue number5
Publication statusPublished - Oct 2002

Fingerprint

Aphids
Malus
aphid
linkage groups
Aphidoidea
loci
clone
Dysaphis
Clone Cells
clones
leaf curling
gene
telomeres
Telomere
pressing
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Leucine
Genetic Recombination
positioning

Keywords

  • Animals
  • Aphids
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Artificial, Bacterial
  • Chromosomes, Plant
  • DNA Primers
  • Genes, Plant
  • Genetic Linkage
  • Genetic Markers
  • In Situ Hybridization, Fluorescence
  • Malus
  • Plant Diseases
  • Telomere
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Resolving the aphid resistance locus Sd-1 on a BAC contig within a sub-telomeric region of Malus linkage group 7. / Cevik, Volkan; King, Graham J.

In: Genome Biology, Vol. 45, No. 5, 10.2002, p. 939-945.

Research output: Contribution to journalArticle

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