Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria

Kai Cheng Yan, Naing Thet, Rachel A. Heylen, Adam C. Sedgwick, Tony D. James, A. Toby A. Jenkins, Xiao Peng He

Research output: Contribution to journalArticlepeer-review

1 Citation (SciVal)

Abstract

Fluorescent probes are extensively used with ever-increasing functions for biological and medical applications. To further solve problems associated with tissue damage, long-wavelength fluorophores have been proposed for the construction of sensors with decreased energy requirements for activation and increased biocompatibility. As such we have repurposed an effective and biologically appliable probe TCF-Bpin combining boronate activating groups and a TCF-OH fluorophore, for monitoring the production of peroxynitrite (ONOO) by bacteria upon treatment with antibiotics. In addition, externally added ONOO was intracellularly visualized, which makes not only the detection of oxidative stress in bacteria possible but also increases the understanding of the mechanisms.

Original languageEnglish
Pages (from-to)1181-1185
Number of pages5
JournalSensors and Diagnostics
Volume2
Issue number2
Early online date13 Jul 2023
DOIs
Publication statusPublished - 1 Sept 2023

Bibliographical note

Funding Information:
XPH wishes to thank the Natural National Science Foundation of China (No. 92253306), Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, the Fundamental Research Funds for the Central Universities (222201717003) and the Programme of Introducing Talents of Discipline to Universities (B16017) for financial support. ATAJ would like to acknowledge funding from the EPSRC, grant reference: EP/V00462X/1. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. RAH thanks the Annette Trust and EPSRC for funding. ACS would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support.

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Analytical Chemistry

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