Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria

Kai Cheng Yan; Naing Thet; Rachel A. Heylen; Adam C. Sedgwick; Tony D. James; A. Toby A. Jenkins; Xiao Peng He

doi:10.1039/d3sd00049d

Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria

Kai Cheng Yan, Naing Thet, Rachel A. Heylen, Adam C. Sedgwick, Tony D. James, A. Toby A. Jenkins, Xiao Peng He

Research output: Contribution to journal › Article › peer-review

2 Citations (SciVal)

Abstract

Fluorescent probes are extensively used with ever-increasing functions for biological and medical applications. To further solve problems associated with tissue damage, long-wavelength fluorophores have been proposed for the construction of sensors with decreased energy requirements for activation and increased biocompatibility. As such we have repurposed an effective and biologically appliable probe TCF-Bpin combining boronate activating groups and a TCF-OH fluorophore, for monitoring the production of peroxynitrite (ONOO⁻) by bacteria upon treatment with antibiotics. In addition, externally added ONOO⁻ was intracellularly visualized, which makes not only the detection of oxidative stress in bacteria possible but also increases the understanding of the mechanisms.

Original language	English
Pages (from-to)	1181-1185
Number of pages	5
Journal	Sensors and Diagnostics
Volume	2
Issue number	2
Early online date	13 Jul 2023
DOIs	https://doi.org/10.1039/d3sd00049d
Publication status	Published - 1 Sept 2023

Bibliographical note

Funding Information:
XPH wishes to thank the Natural National Science Foundation of China (No. 92253306), Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, the Fundamental Research Funds for the Central Universities (222201717003) and the Programme of Introducing Talents of Discipline to Universities (B16017) for financial support. ATAJ would like to acknowledge funding from the EPSRC, grant reference: EP/V00462X/1. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. RAH thanks the Annette Trust and EPSRC for funding. ACS would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support.

Funding

XPH wishes to thank the Natural National Science Foundation of China (No. 92253306), Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, the Fundamental Research Funds for the Central Universities (222201717003) and the Programme of Introducing Talents of Discipline to Universities (B16017) for financial support. ATAJ would like to acknowledge funding from the EPSRC, grant reference: EP/V00462X/1. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. RAH thanks the Annette Trust and EPSRC for funding. ACS would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support.

ASJC Scopus subject areas

Chemistry (miscellaneous)
Analytical Chemistry

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10.1039/d3sd00049dLicence: CC BY

Cite this

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title = "Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria",

abstract = "Fluorescent probes are extensively used with ever-increasing functions for biological and medical applications. To further solve problems associated with tissue damage, long-wavelength fluorophores have been proposed for the construction of sensors with decreased energy requirements for activation and increased biocompatibility. As such we have repurposed an effective and biologically appliable probe TCF-Bpin combining boronate activating groups and a TCF-OH fluorophore, for monitoring the production of peroxynitrite (ONOO−) by bacteria upon treatment with antibiotics. In addition, externally added ONOO− was intracellularly visualized, which makes not only the detection of oxidative stress in bacteria possible but also increases the understanding of the mechanisms.",

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AU - Jenkins, A. Toby A.

AU - He, Xiao Peng

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Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

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Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

Cite this