Abstract
We have investigated actions of the Wilms' tumour suppressor zinc finger transcription factor, WT1, on promoters of the mouse insulin-like growth factor II-encoding gene (Igf-2). Two variant forms of WT1 repressed the two major Igf-2 promoters (P2 and P3) in transient transfection assays. WT1-binding sites were characterised in both these promoters and in the transcribed region downstream from P2, exon 2. In each of these regions, there was a pair of WT1-binding sites, and mutational analysis of the exon-2 sites indicated that both were required for full repression. Cooperative binding of WT1 to these sites might explain the dominant-negative mutations of WT1 observed in some Wilms' tumours and Denys-Drash syndrome cases.
Original language | English |
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Pages (from-to) | 239-43 |
Number of pages | 5 |
Journal | Gene |
Volume | 167 |
Issue number | 1-2 |
Publication status | Published - 29 Dec 1995 |
Keywords
- Animals
- Base Sequence
- Binding Sites
- Consensus Sequence
- DNA-Binding Proteins
- Gene Expression Regulation
- Insulin-Like Growth Factor II
- Mice
- Molecular Sequence Data
- Promoter Regions, Genetic
- RNA, Messenger
- Repressor Proteins
- Transcription Factors
- WT1 Proteins
- Journal Article
- Research Support, Non-U.S. Gov't