Repression of promoters for the mouse insulin-like growth factor II-encoding gene (Igf-2) by products of the Wilms' tumour suppressor gene wt1

A Ward, J A Pooler, K Miyagawa, Antonio Duarte, N D Hastie, A A Caricasole

Research output: Contribution to journalArticle

Abstract

We have investigated actions of the Wilms' tumour suppressor zinc finger transcription factor, WT1, on promoters of the mouse insulin-like growth factor II-encoding gene (Igf-2). Two variant forms of WT1 repressed the two major Igf-2 promoters (P2 and P3) in transient transfection assays. WT1-binding sites were characterised in both these promoters and in the transcribed region downstream from P2, exon 2. In each of these regions, there was a pair of WT1-binding sites, and mutational analysis of the exon-2 sites indicated that both were required for full repression. Cooperative binding of WT1 to these sites might explain the dominant-negative mutations of WT1 observed in some Wilms' tumours and Denys-Drash syndrome cases.

Original languageEnglish
Pages (from-to)239-43
Number of pages5
JournalGene
Volume167
Issue number1-2
Publication statusPublished - 29 Dec 1995

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Keywords

  • Animals
  • Base Sequence
  • Binding Sites
  • Consensus Sequence
  • DNA-Binding Proteins
  • Gene Expression Regulation
  • Insulin-Like Growth Factor II
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA, Messenger
  • Repressor Proteins
  • Transcription Factors
  • WT1 Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Ward, A., Pooler, J. A., Miyagawa, K., Duarte, A., Hastie, N. D., & Caricasole, A. A. (1995). Repression of promoters for the mouse insulin-like growth factor II-encoding gene (Igf-2) by products of the Wilms' tumour suppressor gene wt1. Gene, 167(1-2), 239-43.