TY - JOUR
T1 - Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure
AU - ESCV Network on Next-Generation Sequencing
AU - Lopez-Labrador, F. Xavier
AU - Brown, Julianne R.
AU - Fischer, Nicole
AU - Harvala, Heli
AU - Van Boheemen, Sander
AU - Cinek, Ondrej
AU - Sayiner, Arzu
AU - Vasehus Madsen, Tina
AU - Auvinen, Eeva
AU - Kufner, Verena
AU - Huber, Michael
AU - Rodriguez, Christophe
AU - Jonges, Marcel
AU - Honemann, Mario
AU - Susi, Petri
AU - Sousa, Hugo
AU - Klapper, Paul E.
AU - Perez-Cataluna, Alba
AU - Hernandez, Marta
AU - Molenkamp, Richard
AU - van der Hoek, Lisa
AU - Schuurman, Rob
AU - Monge Gomes Do Couto, Natacha
AU - Leuzinger, Karoline
AU - Simmonds, Peter
AU - Beer, Martin
AU - Hoper, Dirk
AU - Kamminga, Sergio
AU - Feltkamp, Mariet C. W.
AU - Rodriguez-Diaz, Jesus
AU - Keyaerts, Els
AU - Chen Nielsen, Xiahui
AU - Puchhammer-Stockl, Elisabeth
AU - Kroes, Aloys C. M.
AU - Buesa, Javier
AU - Breuer, Judy
AU - Claas, Eric C. J.
AU - de Vries, Jutte J. C.
PY - 2021/1/31
Y1 - 2021/1/31
N2 - Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
AB - Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
KW - Viral metagenomics
KW - High-throughput sequencing
KW - Next-generation sequencing
KW - Wet lab
KW - Recommendations
U2 - 10.1016/j.jcv.2020.104691
DO - 10.1016/j.jcv.2020.104691
M3 - Article
SN - 1386-6532
VL - 134
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
M1 - 104691
ER -