Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

Xiaolong Sun, Karel Lacina, Elena C. Ramsamy, Stephen E. Flower, John S. Fossey, Xuhong Qian, Eric V. Anslyn, Steven D. Bull, Tony D. James

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Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS–NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B–N interaction. Our simple system produces a visible colorimetric change and on–off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).
Original languageEnglish
Pages (from-to)2963-2967
Number of pages5
JournalChemical Science
Issue number5
Early online date6 Mar 2015
Publication statusPublished - 1 May 2015

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