Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.
- Anti-Bacterial Agents/chemistry
- Biofilms/drug effects
- Escherichia coli/drug effects
- Hydrogen Peroxide/chemistry
- Methicillin-Resistant Staphylococcus aureus/physiology
- Microbial Sensitivity Tests
- Pseudomonas aeruginosa/drug effects