Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Bethany L. Patenall, George T. Williams‡, Lauren Gwynne, Liam J. Stephens, Emma V. Lampard, Hollie J. Hathaway, Naing T. Thet, Amber E. Young, Mark J. Sutton, Robert D. Short, Steven D. Bull, Tony D. James, Adam C. Sedgwick, A. Toby A. Jenkins

Research output: Contribution to journalArticle

Abstract

Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.

Original languageEnglish
Pages (from-to)15129-15132
Number of pages4
JournalChemical Communications
Volume55
Issue number100
Early online date2 Dec 2019
DOIs
Publication statusPublished - 12 Dec 2019

Keywords

  • Anthraquinones/chemistry
  • Anti-Bacterial Agents/chemistry
  • Biofilms/drug effects
  • Escherichia coli/drug effects
  • Hydrogels/chemistry
  • Hydrogen Peroxide/chemistry
  • Methicillin-Resistant Staphylococcus aureus/physiology
  • Microbial Sensitivity Tests
  • Pseudomonas aeruginosa/drug effects
  • Solubility

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