Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Bethany L. Patenall; George T. Williams‡; Lauren Gwynne; Liam J. Stephens; Emma V. Lampard; Hollie J. Hathaway; Naing T. Thet; Amber E. Young; Mark J. Sutton; Robert D. Short; Steven D. Bull; Tony D. James; Adam C. Sedgwick; A. Toby A. Jenkins

doi:10.1039/c9cc07759f

Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Bethany L. Patenall, George T. Williams‡, Lauren Gwynne, Liam J. Stephens, Emma V. Lampard, Hollie J. Hathaway, Naing T. Thet, Amber E. Young, Mark J. Sutton, Robert D. Short, Steven D. Bull, Tony D. James, Adam C. Sedgwick, A. Toby A. Jenkins

Research output: Contribution to journal › Article › peer-review

13 Citations (SciVal)

Abstract

Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H₂O₂) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H₂O₂, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H₂O₂-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.

Original language	English
Pages (from-to)	15129-15132
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	100
Early online date	2 Dec 2019
DOIs	https://doi.org/10.1039/c9cc07759f
Publication status	Published - 12 Dec 2019

Keywords

Anthraquinones/chemistry
Anti-Bacterial Agents/chemistry
Biofilms/drug effects
Escherichia coli/drug effects
Hydrogels/chemistry
Hydrogen Peroxide/chemistry
Methicillin-Resistant Staphylococcus aureus/physiology
Microbial Sensitivity Tests
Pseudomonas aeruginosa/drug effects
Solubility

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Patenall, B. L., Williams‡, G. T., Gwynne, L., Stephens, L. J., Lampard, E. V., Hathaway, H. J., Thet, N. T., Young, A. E., Sutton, M. J., Short, R. D., Bull, S. D., James, T. D., Sedgwick, A. C., & Jenkins, A. T. A. (2019). Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition. Chemical Communications, 55(100), 15129-15132. https://doi.org/10.1039/c9cc07759f

Patenall, BL, Williams‡, GT, Gwynne, L, Stephens, LJ, Lampard, EV, Hathaway, HJ, Thet, NT, Young, AE, Sutton, MJ, Short, RD, Bull, SD, James, TD, Sedgwick, AC & Jenkins, ATA 2019, 'Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition', Chemical Communications, vol. 55, no. 100, pp. 15129-15132. https://doi.org/10.1039/c9cc07759f

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title = "Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition",

abstract = "Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.",

keywords = "Anthraquinones/chemistry, Anti-Bacterial Agents/chemistry, Biofilms/drug effects, Escherichia coli/drug effects, Hydrogels/chemistry, Hydrogen Peroxide/chemistry, Methicillin-Resistant Staphylococcus aureus/physiology, Microbial Sensitivity Tests, Pseudomonas aeruginosa/drug effects, Solubility",

author = "Patenall, {Bethany L.} and Williams‡, {George T.} and Lauren Gwynne and Stephens, {Liam J.} and Lampard, {Emma V.} and Hathaway, {Hollie J.} and Thet, {Naing T.} and Young, {Amber E.} and Sutton, {Mark J.} and Short, {Robert D.} and Bull, {Steven D.} and James, {Tony D.} and Sedgwick, {Adam C.} and Jenkins, {A. Toby A.}",

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doi = "10.1039/c9cc07759f",

language = "English",

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AU - Patenall, Bethany L.

AU - Williams‡, George T.

AU - Gwynne, Lauren

AU - Stephens, Liam J.

AU - Lampard, Emma V.

AU - Hathaway, Hollie J.

AU - Thet, Naing T.

AU - Young, Amber E.

AU - Sutton, Mark J.

AU - Short, Robert D.

AU - Bull, Steven D.

AU - James, Tony D.

AU - Sedgwick, Adam C.

AU - Jenkins, A. Toby A.

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AB - Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.

KW - Anthraquinones/chemistry

KW - Anti-Bacterial Agents/chemistry

KW - Biofilms/drug effects

KW - Escherichia coli/drug effects

KW - Hydrogels/chemistry

KW - Hydrogen Peroxide/chemistry

KW - Methicillin-Resistant Staphylococcus aureus/physiology

KW - Microbial Sensitivity Tests

KW - Pseudomonas aeruginosa/drug effects

KW - Solubility

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DO - 10.1039/c9cc07759f

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AN - SCOPUS:85076446924

SN - 1359-7345

VL - 55

SP - 15129

EP - 15132

JO - Chemical Communications

JF - Chemical Communications

IS - 100

ER -

Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

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Keywords

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Smartwound-plasma

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Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Abstract

Keywords

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Smartwound-plasma

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