Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Xiaolong Sun, Maria L. Odyniec, Adam C. Sedgwick, Karel Lacina, Suying Xu, Taotao Qiang, Steven D. Bull, Frank Marken, Tony D. James

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on-off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.

Original languageEnglish
Pages (from-to)1058-1062
Number of pages5
JournalOrganic Chemistry Frontiers
Volume4
Issue number6
Early online date17 Feb 2017
DOIs
Publication statusPublished - 1 Jun 2017

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Hydrogen Peroxide
Assays
Phenol
Complexation
Fluorescence
Color
Alizarin Red S
benzeneboronic acid

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide. / Sun, Xiaolong; Odyniec, Maria L.; Sedgwick, Adam C.; Lacina, Karel; Xu, Suying; Qiang, Taotao; Bull, Steven D.; Marken, Frank; James, Tony D.

In: Organic Chemistry Frontiers, Vol. 4, No. 6, 01.06.2017, p. 1058-1062.

Research output: Contribution to journalArticle

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AU - Xu, Suying

AU - Qiang, Taotao

AU - Bull, Steven D.

AU - Marken, Frank

AU - James, Tony D.

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