Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H₂O₂) in aqueous media. This platform has demonstrated its ability to detect H₂O₂via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on-off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H₂O₂. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H₂O₂ and hydrogen peroxide-related species for environmental and physiological detection.