Ratiometric electrochemical detection of hydrogen peroxide and glucose

Sean Goggins, Ellen A. Apsey, Mary F. Mahon, Christopher G. Frost

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) detection is of high importance as it is a versatile (bio)marker whose detection can indicate the presence of explosives, enzyme activity and cell signalling pathways. Herein, we demonstrate the rapid and accurate ratiometric electrochemical detection of H2O2 using disposable screen-printed electrodes through a reaction-based indicator assay. Ferrocene derivatives equipped with self-immolative linkers and boronic acid ester moieties were synthesised and tested, and, through a thorough assay optimisation, the optimum probe showed good stability, sensitivity and selectivity towards H2O2. The optimised conditions were then applied to the indirect detection of glucose via an enzymatic assay, capable of distinguishing 10 μM from the background within minutes.

Original languageEnglish
Pages (from-to)2459-2466
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume15
Issue number11
DOIs
Publication statusPublished - 21 Feb 2017

Fingerprint

Boronic Acids
Enzyme Assays
hydrogen peroxide
glucose
Hydrogen Peroxide
Assays
Electrodes
Esters
Glucose
Enzymes
Cell signaling
Enzyme activity
enzyme activity
markers
Derivatives
esters
selectivity
acids
optimization
electrodes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Ratiometric electrochemical detection of hydrogen peroxide and glucose. / Goggins, Sean; Apsey, Ellen A.; Mahon, Mary F.; Frost, Christopher G.

In: Organic and Biomolecular Chemistry, Vol. 15, No. 11, 21.02.2017, p. 2459-2466.

Research output: Contribution to journalArticle

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