Projects per year
Abstract
Current sequence databases now contain numerous whole genome sequences of pathogenic bacteria. However, many of the predicted genes lack any functional annotation. We describe an assumption-free approach, Rapid Virulence Annotation (RVA), for the high-throughput parallel screening of genomic libraries against four different taxa: insects, nematodes, amoeba, and mammalian macrophages. These hosts represent different aspects of both the vertebrate and invertebrate immune system. Here, we apply RVA to the emerging human pathogen Photorhabdus asymbiotica using “gain of toxicity” assays of recombinant Escherichia coli clones. We describe a wealth of potential virulence loci and attribute biological function to several putative genomic islands, which may then be further characterized using conventional molecular techniques. The application of RVA to other pathogen genomes promises to ascribe biological function to otherwise uncharacterized virulence genes.
Original language | English |
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Pages (from-to) | 15967-15972 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 105 |
Issue number | 41 |
Early online date | 5 Oct 2008 |
DOIs | |
Publication status | Published - 14 Oct 2008 |
Bibliographical note
Correction published in PNAS, 106(6), 2083-2083. DOI: 10.1073/pnas.0811896106Fingerprint
Dive into the research topics of 'Rapid Virulence Annotation (RVA): identification of virulence factors using a bacterial genome library and multiple invertebrate hosts'. Together they form a unique fingerprint.Projects
- 3 Finished
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Modelling Systems for Managing Bee Disease: The Epidermiology of European Foul Brood
Feil, E. (PI), Britton, N. (CoI) & Priest, N. (CoI)
Biotechnology and Biological Sciences Research Council
1/11/10 → 31/10/13
Project: Research council
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RAPID BACTERIAL VIRULENCE ANNOTATION FOR THE POST GENOMIC ER A
Waterfield, N. (PI)
Biotechnology and Biological Sciences Research Council
1/07/07 → 30/09/10
Project: Research council
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A NOVEL TOXIN DELIVERY SYSTEM IN BACTERIA
Waterfield, N. (PI)
Biotechnology and Biological Sciences Research Council
21/11/05 → 6/08/09
Project: Research council