Projects per year
Abstract
The Rab-GTPase-activating proteins (GAPs) TBC1D1 and TBC1D4 play important roles in the insulin-stimulated translocation of the glucose transporter GLUT4 from intracellular vesicles to the plasma membrane in muscle cells and adipocytes. We identified Rab28 as a substrate for the GAP domains of both TBC1D1 and TBC1D4 in vitro. Rab28 is expressed in adipose cells and skeletal muscle, and its GTP-binding state is acutely regulated by insulin. We found that in intact isolated mouse skeletal muscle, siRNA-mediated knockdown of Rab28 decreases basal glucose uptake. Conversely, in primary rat adipose cells, overexpression of Rab28-Q72L, a constitutively active mutant, increases basal cell surface levels of an epitope-tagged HA-GLUT4. Our results indicate that Rab28 is a novel GTPase involved in the intracellular retention of GLUT4 in insulin target cells.
Original language | English |
---|---|
Pages (from-to) | 88-96 |
Number of pages | 9 |
Journal | FEBS Letters |
Volume | 591 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jan 2017 |
Keywords
- adipose tissue
- GLUT4
- GTPase
- insulin signaling
- skeletal muscle
Fingerprint
Dive into the research topics of 'Rab28 is a TBC1D1/TBC1D4 substrate involved in GLUT4 trafficking'. Together they form a unique fingerprint.Projects
- 4 Finished
-
Elucidation of Final Stages in Coupling of Insulin Signalling to CLUT4 Translocation
Holman, G. (PI) & Koumanov, F. (CoI)
1/07/12 → 30/06/15
Project: Research council
-
Role of TBC11D1 in Heart Cell Insulin Resistance
Holman, G. (PI) & Koumanov, F. (CoI)
1/09/11 → 30/11/13
Project: UK charity
-
COOPERATIVE GROUP IN ORGANOGENESIS, GROWTH AND REGENERATION
Ward, A. (PI), Holman, G. (CoI), Hurst, L. (CoI), Kelsh, R. (CoI), Slack, J. (CoI) & Tosh, D. (CoI)
21/06/04 → 20/06/09
Project: Research council