Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence

F Giuntini; L Bourre; A J MacRobert; M Wilson; Ian M Eggleston

doi:10.1016/j.jchromb.2008.09.031

Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence

F Giuntini, L Bourre, A J MacRobert, M Wilson, Ian M Eggleston

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.

Original language	English
Pages (from-to)	562-566
Number of pages	5
Journal	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume	875
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2008.09.031
Publication status	Published - 15 Nov 2008

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10.1016/j.jchromb.2008.09.031

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@article{9beece08cac349d0bfe87cf31fb91fba,

title = "Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence",

abstract = "The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.",

author = "F Giuntini and L Bourre and MacRobert, \{A J\} and M Wilson and Eggleston, \{Ian M\}",

year = "2008",

month = nov,

day = "15",

doi = "10.1016/j.jchromb.2008.09.031",

language = "English",

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journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",

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publisher = "Elsevier B.V.",

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T1 - Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence

AU - Giuntini, F

AU - Bourre, L

AU - MacRobert, A J

AU - Wilson, M

AU - Eggleston, Ian M

PY - 2008/11/15

Y1 - 2008/11/15

N2 - The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.

AB - The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.

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UR - http://dx.doi.org/10.1016/j.jchromb.2008.09.031

U2 - 10.1016/j.jchromb.2008.09.031

DO - 10.1016/j.jchromb.2008.09.031

M3 - Article

SN - 1570-0232

VL - 875

SP - 562

EP - 566

JO - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence

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IMPROVING THE DELIVERY OF 5-AMINOLAEVULINIC ACID IN PHOTODYN AMIC THERAPY

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Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence

Abstract

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IMPROVING THE DELIVERY OF 5-AMINOLAEVULINIC ACID IN PHOTODYN AMIC THERAPY

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