Projects per year
The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.
|Number of pages||5|
|Journal||Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences|
|Publication status||Published - 15 Nov 2008|
FingerprintDive into the research topics of 'Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence'. Together they form a unique fingerprint.
- 1 Finished
26/07/06 → 25/07/09
Project: Research council