Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence

F Giuntini, L Bourre, A J MacRobert, M Wilson, Ian M Eggleston

Research output: Contribution to journalArticle

Abstract

The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.
Original languageEnglish
Pages (from-to)562-566
Number of pages5
JournalJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume875
Issue number2
DOIs
Publication statusPublished - 15 Nov 2008

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