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The development of a reliable sensitive method for the HPLC determination of 5-aminolaevulinic acid (ALA) and ALA esters in cell lysates is described. The method relies on the quantification of a fluorescent derivative of ALA following its derivatisation with acetylacetone and formaldehyde. Following this procedure it is possible to quantify ALA in cell lysates with no need for pre-purification of the sample. The method has been validated in two ranges of concentration (0.6-65 mu M, 0.1-10 mu g/mL, and 30-600 mu M, 5-100 mu g/mL), and has also been extended and validated for the determination of ALA released from ALA prodrugs after acidic hydrolysis.
|Number of pages||5|
|Journal||Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences|
|Publication status||Published - 15 Nov 2008|
Giuntini, F., Bourre, L., MacRobert, A. J., Wilson, M., & Eggleston, I. M. (2008). Quantitative determination of 5-aminolaevulinic acid and its esters in cell lysates by HPLC-fluorescence. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 875(2), 562-566. https://doi.org/10.1016/j.jchromb.2008.09.031