Quantitative chromosome conformation capture

Raffaella Nativio, Yoko Ito, Adele Murrell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

It is becoming increasingly apparent that chromatin is not randomly folded into the nucleus, but instead is highly organized into specific conformations within the nucleus. One consequence of such higher order structure is that chromatin looping can bring together genomic elements which are separated by several hundreds of kilobases, such as enhancers and promoters, and functionally facilitate their interaction. The Chromosome Conformation Capture (3C) assay is a powerful technique to detect looping structures and assess the probability of interaction between distant genomic elements (1-3). Here we describe the 3C methodology, its power, and limitations, together with the controls and normalization steps required for an accurate analysis.
Original languageEnglish
Pages (from-to)173-185
Number of pages13
JournalMethods in Molecular Biology
Volume925
DOIs
Publication statusPublished - 2012

Keywords

  • Animals
  • Cell Nucleus
  • Chromatin
  • DNA
  • Humans
  • Mice
  • Nucleic Acid Conformation
  • Protein Conformation

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