Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss; Kathryn Woodfine; Joanna E. Huddleston; Adele Murrell; Anthony J. Rutherford; Kay Elder; Amir Ali Khan; Karen Hemmings; Helen Picton

doi:10.1016/j.fertnstert.2011.04.035

Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss, Kathryn Woodfine, Joanna E. Huddleston, Adele Murrell, Anthony J. Rutherford, Kay Elder, Amir Ali Khan, Karen Hemmings, Helen Picton

Research output: Contribution to journal › Article › peer-review

12 Citations (SciVal)

Abstract

We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

Original language	English
Pages (from-to)	2564-2567.e8
Journal	Fertility and Sterility
Volume	95
Issue number	8
DOIs	https://doi.org/10.1016/j.fertnstert.2011.04.035
Publication status	Published - 30 Jun 2011

Keywords

assisted reproduction
blastocyst
embryonic stem cells
epigenetic
Genomic imprinting
methylation
preimplantation

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology

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10.1016/j.fertnstert.2011.04.035

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title = "Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing",

abstract = "We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.",

keywords = "assisted reproduction, blastocyst, embryonic stem cells, epigenetic, Genomic imprinting, methylation, preimplantation",

author = "John Huntriss and Kathryn Woodfine and Huddleston, \{Joanna E.\} and Adele Murrell and Rutherford, \{Anthony J.\} and Kay Elder and Khan, \{Amir Ali\} and Karen Hemmings and Helen Picton",

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T1 - Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

AU - Huntriss, John

AU - Woodfine, Kathryn

AU - Huddleston, Joanna E.

AU - Murrell, Adele

AU - Rutherford, Anthony J.

AU - Elder, Kay

AU - Khan, Amir Ali

AU - Hemmings, Karen

AU - Picton, Helen

PY - 2011/6/30

Y1 - 2011/6/30

N2 - We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

AB - We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

KW - assisted reproduction

KW - blastocyst

KW - embryonic stem cells

KW - epigenetic

KW - Genomic imprinting

KW - methylation

KW - preimplantation

UR - https://www.scopus.com/pages/publications/2-s2.0-79959690847

U2 - 10.1016/j.fertnstert.2011.04.035

DO - 10.1016/j.fertnstert.2011.04.035

M3 - Article

C2 - 21575943

SN - 0015-0282

VL - 95

SP - 2564-2567.e8

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 8

ER -

Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

Abstract

Keywords

ASJC Scopus subject areas

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