Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss; Kathryn Woodfine; Joanna E. Huddleston; Adele Murrell; Anthony J. Rutherford; Kay Elder; Amir Ali Khan; Karen Hemmings; Helen Picton

doi:10.1016/j.fertnstert.2011.04.035

Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss, Kathryn Woodfine, Joanna E. Huddleston, Adele Murrell, Anthony J. Rutherford, Kay Elder, Amir Ali Khan, Karen Hemmings, Helen Picton

Research output: Contribution to journal › Article › peer-review

11 Citations (SciVal)

Abstract

We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

Original language	English
Pages (from-to)	2564-2567.e8
Journal	Fertility and Sterility
Volume	95
Issue number	8
DOIs	https://doi.org/10.1016/j.fertnstert.2011.04.035 https://doi.org/10.1016/j.fertnstert.2011.04.035
Publication status	Published - 30 Jun 2011

Keywords

assisted reproduction
blastocyst
embryonic stem cells
epigenetic
Genomic imprinting
methylation
preimplantation

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology

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Huntriss, J., Woodfine, K., Huddleston, J. E., Murrell, A., Rutherford, A. J., Elder, K., Khan, A. A., Hemmings, K., & Picton, H. (2011). Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing. Fertility and Sterility, 95(8), 2564-2567.e8. https://doi.org/10.1016/j.fertnstert.2011.04.035, https://doi.org/10.1016/j.fertnstert.2011.04.035

Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing. / Huntriss, John; Woodfine, Kathryn; Huddleston, Joanna E.; Murrell, Adele; Rutherford, Anthony J.; Elder, Kay; Khan, Amir Ali; Hemmings, Karen; Picton, Helen.

In: Fertility and Sterility, Vol. 95, No. 8, 30.06.2011, p. 2564-2567.e8.

Research output: Contribution to journal › Article › peer-review

Huntriss, J, Woodfine, K, Huddleston, JE, Murrell, A, Rutherford, AJ, Elder, K, Khan, AA, Hemmings, K & Picton, H 2011, 'Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing', Fertility and Sterility, vol. 95, no. 8, pp. 2564-2567.e8. https://doi.org/10.1016/j.fertnstert.2011.04.035, https://doi.org/10.1016/j.fertnstert.2011.04.035

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abstract = "We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.",

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AU - Elder, Kay

AU - Khan, Amir Ali

AU - Hemmings, Karen

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Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

Abstract

Keywords

ASJC Scopus subject areas

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