Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss; Kathryn Woodfine; Joanna E. Huddleston; Adele Murrell; Anthony J. Rutherford; Kay Elder; Amir Ali Khan; Karen Hemmings; Helen Picton

doi:10.1016/j.fertnstert.2011.04.035

Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss, Kathryn Woodfine, Joanna E. Huddleston, Adele Murrell, Anthony J. Rutherford, Kay Elder, Amir Ali Khan, Karen Hemmings, Helen Picton

Department of Biology & Biochemistry

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

Original language	English
Pages (from-to)	2564-2567.e8
Journal	Fertility and Sterility
Volume	95
Issue number	8
DOIs	https://doi.org/10.1016/j.fertnstert.2011.04.035
Publication status	Published - 30 Jun 2011

Keywords

Blastocyst
Cell Line
DNA Methylation
Embryo Culture Techniques
Embryonic Stem Cells
GRB10 Adaptor Protein
Gene Expression Regulation, Developmental
Genomic Imprinting
Humans
Potassium Channels, Voltage-Gated
Preimplantation Diagnosis
Proteins
RNA, Long Noncoding
Retinoblastoma Protein
Sequence Analysis, DNA
snRNP Core Proteins

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10.1016/j.fertnstert.2011.04.035

http://dx.doi.org/10.1016/j.fertnstert.2011.04.035

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Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing. / Huntriss, John; Woodfine, Kathryn; Huddleston, Joanna E.; Murrell, Adele; Rutherford, Anthony J.; Elder, Kay; Khan, Amir Ali; Hemmings, Karen; Picton, Helen.

In: Fertility and Sterility, Vol. 95, No. 8, 30.06.2011, p. 2564-2567.e8.

Research output: Contribution to journal › Article › peer-review

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abstract = "We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.",

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author = "John Huntriss and Kathryn Woodfine and Huddleston, {Joanna E.} and Adele Murrell and Rutherford, {Anthony J.} and Kay Elder and Khan, {Amir Ali} and Karen Hemmings and Helen Picton",

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AU - Rutherford, Anthony J.

AU - Elder, Kay

AU - Khan, Amir Ali

AU - Hemmings, Karen

AU - Picton, Helen

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AB - We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.

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KW - Cell Line

KW - DNA Methylation

KW - Embryo Culture Techniques

KW - Embryonic Stem Cells

KW - GRB10 Adaptor Protein

KW - Gene Expression Regulation, Developmental

KW - Genomic Imprinting

KW - Humans

KW - Potassium Channels, Voltage-Gated

KW - Preimplantation Diagnosis

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KW - RNA, Long Noncoding

KW - Retinoblastoma Protein

KW - Sequence Analysis, DNA

KW - snRNP Core Proteins

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