Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing

John Huntriss, Kathryn Woodfine, Joanna E. Huddleston, Adele Murrell, Anthony J. Rutherford, Kay Elder, Amir Ali Khan, Karen Hemmings, Helen Picton

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.
Original languageEnglish
Pages (from-to)2564-2567.e8
JournalFertility and Sterility
Volume95
Issue number8
DOIs
Publication statusPublished - 30 Jun 2011

Keywords

  • Blastocyst
  • Cell Line
  • DNA Methylation
  • Embryo Culture Techniques
  • Embryonic Stem Cells
  • GRB10 Adaptor Protein
  • Gene Expression Regulation, Developmental
  • Genomic Imprinting
  • Humans
  • Potassium Channels, Voltage-Gated
  • Preimplantation Diagnosis
  • Proteins
  • RNA, Long Noncoding
  • Retinoblastoma Protein
  • Sequence Analysis, DNA
  • snRNP Core Proteins

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