TY - JOUR
T1 - Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing
AU - Huntriss, John
AU - Woodfine, Kathryn
AU - Huddleston, Joanna E.
AU - Murrell, Adele
AU - Rutherford, Anthony J.
AU - Elder, Kay
AU - Khan, Amir Ali
AU - Hemmings, Karen
AU - Picton, Helen
PY - 2011/6/30
Y1 - 2011/6/30
N2 - We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.
AB - We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.
KW - assisted reproduction
KW - blastocyst
KW - embryonic stem cells
KW - epigenetic
KW - Genomic imprinting
KW - methylation
KW - preimplantation
UR - http://www.scopus.com/inward/record.url?scp=79959690847&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2011.04.035
DO - 10.1016/j.fertnstert.2011.04.035
M3 - Article
C2 - 21575943
VL - 95
SP - 2564-2567.e8
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 8
ER -