Abstract
We report the first quantitative assessment of DNA methylation for any gene in the human preimplantation embryo to reveal that imprints exist at KvDMR1, RB1, SNRPN, and GRB10 in the human blastocyst. For comparison, in two human embryonic stem cell lines, imprints were also observed at KvDMR1, SNRPN, GRB10, and other imprinted loci, whereas RB1 and MEG3 were hypermethylated.
Original language | English |
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Pages (from-to) | 2564-2567.e8 |
Journal | Fertility and Sterility |
Volume | 95 |
Issue number | 8 |
DOIs | |
Publication status | Published - 30 Jun 2011 |
Keywords
- Blastocyst
- Cell Line
- DNA Methylation
- Embryo Culture Techniques
- Embryonic Stem Cells
- GRB10 Adaptor Protein
- Gene Expression Regulation, Developmental
- Genomic Imprinting
- Humans
- Potassium Channels, Voltage-Gated
- Preimplantation Diagnosis
- Proteins
- RNA, Long Noncoding
- Retinoblastoma Protein
- Sequence Analysis, DNA
- snRNP Core Proteins