Purified human hematopoietic stem cells contribute to the generation of cardiomyocytes through cell fusion

F Ishikawa, H Shimazu, L D Shultz, M Fukata, R Nakamura, B Lyons, K Shimoda, S Shimoda, T Kanemaru, K I Nakamura, H Ito, Y Kaji, Anthony C F Perry, M Harada

Research output: Contribution to journalArticle

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Abstract

To obtain insights into the cardiomyogenic potential of hematopoietic tissue, we intravenously (i.v.) injected purified hematopoietic stem/progenitor cells into newborn recipients that may fully potentiate the developmental plasticity of stem cells. Transplantation of mouse bone marrow ( BM) lineage antigen-negative (Lin(-)) cells resulted in the generation of the cells that displayed cardiomyocyte- specific antigenic profiles and contractile function when transplanted into syngeneic newborn recipients. To clarify the mechanism underlying the cardiomyogenic potential, green fluorescent protein (GFP)-labeled BM Lin(-)ScaI(+) hematopoietic progenitors were transplanted into neonatal mice constitutively expressing cyan fluorescence protein ( CFP). Lambda image acquisition and linear unmixing analysis using confocal microscopy successfully separated GFP and CFP, and revealed that odonor GFP(+) cardiomyocytes coexpressed host-derived CFP. We further reconstituted human hemopoietic- and immune systems in mice by injecting human cord blood (CB)-derived Lin(-)CD34(+)CD38(-) hematopoietic stem cells (HSCs) into neonatal T cell(-)B cell(-)NK cell(-) immune-deficient NOD/SCID/IL2r gamma(null) mice. Fluoroescence in situ hybridization analysis of recipient cardiac tissues demonstrated that human and murine chromosomes were colocalized in the same cardiomyocytes, indicating that cell fusion occurred between human hematopoietic progeny and mouse cardiomyocytes. These syngeneic- and xenogeneic neonatal transplantations provide compelling evidence that hematopoietic stem/progenitor cells contribute to the postnatal generation of cardiomyocytes through cell fusion, not through transdifferentiation.
Original languageEnglish
Pages (from-to)950-952
Number of pages3
JournalFASEB Journal
Volume20
Issue number7
DOIs
Publication statusPublished - 2006

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Cell Fusion
Hematopoietic Stem Cells
Stem cells
Cardiac Myocytes
Fusion reactions
Green Fluorescent Proteins
Fluorescence
Bone
Tissue
Proteins
T-cells
Confocal microscopy
Immune system
Image acquisition
Chromosomes
Plasticity
Human Chromosomes
Bone Marrow Transplantation
Blood
Fetal Blood

Cite this

Ishikawa, F., Shimazu, H., Shultz, L. D., Fukata, M., Nakamura, R., Lyons, B., ... Harada, M. (2006). Purified human hematopoietic stem cells contribute to the generation of cardiomyocytes through cell fusion. FASEB Journal, 20(7), 950-952. https://doi.org/10.1096/fj.05-4863fje

Purified human hematopoietic stem cells contribute to the generation of cardiomyocytes through cell fusion. / Ishikawa, F; Shimazu, H; Shultz, L D; Fukata, M; Nakamura, R; Lyons, B; Shimoda, K; Shimoda, S; Kanemaru, T; Nakamura, K I; Ito, H; Kaji, Y; Perry, Anthony C F; Harada, M.

In: FASEB Journal, Vol. 20, No. 7, 2006, p. 950-952.

Research output: Contribution to journalArticle

Ishikawa, F, Shimazu, H, Shultz, LD, Fukata, M, Nakamura, R, Lyons, B, Shimoda, K, Shimoda, S, Kanemaru, T, Nakamura, KI, Ito, H, Kaji, Y, Perry, ACF & Harada, M 2006, 'Purified human hematopoietic stem cells contribute to the generation of cardiomyocytes through cell fusion', FASEB Journal, vol. 20, no. 7, pp. 950-952. https://doi.org/10.1096/fj.05-4863fje
Ishikawa, F ; Shimazu, H ; Shultz, L D ; Fukata, M ; Nakamura, R ; Lyons, B ; Shimoda, K ; Shimoda, S ; Kanemaru, T ; Nakamura, K I ; Ito, H ; Kaji, Y ; Perry, Anthony C F ; Harada, M. / Purified human hematopoietic stem cells contribute to the generation of cardiomyocytes through cell fusion. In: FASEB Journal. 2006 ; Vol. 20, No. 7. pp. 950-952.
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