Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus

Edward F. Passalacqua; Rossalyn D. Brehm; K. Ravi Acharya; Howard S. Tranter

doi:10.1016/0022-2836(92)90882-K

Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus

Edward F. Passalacqua, Rossalyn D. Brehm, K. Ravi Acharya, Howard S. Tranter

Research output: Contribution to journal › Article › peer-review

Abstract

High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (>99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C222₁, with unit cell dimensions a = 108·6 A ̊, b = 177·6 A ̊ and c = 97·5 A ̊, with three molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for three-dimensional X-ray structural analysis.

Original language	English
Pages (from-to)	983-986
Number of pages	4
Journal	Journal of Molecular Biology
Volume	228
Issue number	3
DOIs	https://doi.org/10.1016/0022-2836(92)90882-K
Publication status	Published - 5 Dec 1992

Funding

We thank the Science & Engineering Council, U.K. for a CASE award to E.F.P. and Professor A. R. Rees for support and encouragement. We acknowledge the technical expertise of Mr Roger Rhind-Tutt and Miss &son Carter for the fermentation.

Keywords

crystallization
dye ligand chromatography
toxic shock syndrome toxin-1
toxin purification
X-ray diffraction

ASJC Scopus subject areas

Virology

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10.1016/0022-2836(92)90882-K

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AU - Tranter, Howard S.

PY - 1992/12/5

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Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus

Abstract

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Keywords

ASJC Scopus subject areas

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