Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus

Edward F. Passalacqua, Rossalyn D. Brehm, K. Ravi Acharya, Howard S. Tranter

Research output: Contribution to journalArticle

Abstract

High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (>99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C2221, with unit cell dimensions a = 108·6 A ̊, b = 177·6 A ̊ and c = 97·5 A ̊, with three molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for three-dimensional X-ray structural analysis.

Original languageEnglish
Pages (from-to)983-986
Number of pages4
JournalJournal of Molecular Biology
Volume228
Issue number3
DOIs
Publication statusPublished - 5 Dec 1992

Keywords

  • crystallization
  • dye ligand chromatography
  • toxic shock syndrome toxin-1
  • toxin purification
  • X-ray diffraction

ASJC Scopus subject areas

  • Virology

Cite this

Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus. / Passalacqua, Edward F.; Brehm, Rossalyn D.; Acharya, K. Ravi; Tranter, Howard S.

In: Journal of Molecular Biology, Vol. 228, No. 3, 05.12.1992, p. 983-986.

Research output: Contribution to journalArticle

Passalacqua, Edward F. ; Brehm, Rossalyn D. ; Acharya, K. Ravi ; Tranter, Howard S. / Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus. In: Journal of Molecular Biology. 1992 ; Vol. 228, No. 3. pp. 983-986.
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