Abstract
High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (>99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C2221, with unit cell dimensions a = 108·6 A ̊, b = 177·6 A ̊ and c = 97·5 A ̊, with three molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for three-dimensional X-ray structural analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 983-986 |
| Number of pages | 4 |
| Journal | Journal of Molecular Biology |
| Volume | 228 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 5 Dec 1992 |
Keywords
- crystallization
- dye ligand chromatography
- toxic shock syndrome toxin-1
- toxin purification
- X-ray diffraction
ASJC Scopus subject areas
- Virology
Cite this
Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus. / Passalacqua, Edward F.; Brehm, Rossalyn D.; Acharya, K. Ravi; Tranter, Howard S.
In: Journal of Molecular Biology, Vol. 228, No. 3, 05.12.1992, p. 983-986.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus
AU - Passalacqua, Edward F.
AU - Brehm, Rossalyn D.
AU - Acharya, K. Ravi
AU - Tranter, Howard S.
PY - 1992/12/5
Y1 - 1992/12/5
N2 - High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (>99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C2221, with unit cell dimensions a = 108·6 A ̊, b = 177·6 A ̊ and c = 97·5 A ̊, with three molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for three-dimensional X-ray structural analysis.
AB - High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (>99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C2221, with unit cell dimensions a = 108·6 A ̊, b = 177·6 A ̊ and c = 97·5 A ̊, with three molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for three-dimensional X-ray structural analysis.
KW - crystallization
KW - dye ligand chromatography
KW - toxic shock syndrome toxin-1
KW - toxin purification
KW - X-ray diffraction
UR - http://www.scopus.com/inward/record.url?scp=0027050832&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(92)90882-K
DO - 10.1016/0022-2836(92)90882-K
M3 - Article
VL - 228
SP - 983
EP - 986
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 3
ER -