Abstract
High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (>99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C2221, with unit cell dimensions a = 108·6 A ̊, b = 177·6 A ̊ and c = 97·5 A ̊, with three molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for three-dimensional X-ray structural analysis.
Original language | English |
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Pages (from-to) | 983-986 |
Number of pages | 4 |
Journal | Journal of Molecular Biology |
Volume | 228 |
Issue number | 3 |
DOIs | |
Publication status | Published - 5 Dec 1992 |
Funding
We thank the Science & Engineering Council, U.K. for a CASE award to E.F.P. and Professor A. R. Rees for support and encouragement. We acknowledge the technical expertise of Mr Roger Rhind-Tutt and Miss &son Carter for the fermentation.
Keywords
- crystallization
- dye ligand chromatography
- toxic shock syndrome toxin-1
- toxin purification
- X-ray diffraction
ASJC Scopus subject areas
- Virology