Projects per year
Abstract
The early detection of wound infection in-situ can dramatically improve patient care pathways and clinical outcomes. There is increasing evidence that within an infected wound the main bacterial mode of living is a biofilm: a confluent community of adherent bacteria encased in an extracellular polymeric matrix. Here we have reported the development of a prototype wound dressing, which switches on a fluorescent color when in contact with pathogenic wound biofilms. The dressing is made of a hydrated agarose film in which the fluorescent dye containing vesicles were mixed with agarose and dispersed within the hydrogel matrix. The static and dynamic models of wound biofilms, from clinical strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis, were established on nano-porous polycarbonate membrane for 24, 48 and 72 hours, and the dressing response to the biofilms on the prototype dressing evaluated. The dressing indicated a clear fluorescent / color response within four hours, only observed when in contact with biofilms produced by a pathogenic strain. The sensitivity of the dressing to biofilms was dependent on the species and strain types of the bacterial pathogens involved, but a relatively higher response was observed in strains considered
good biofilm formers. There was a clear difference in the levels of dressing response, when
dressings were tested on bacteria grown in biofilm or in planktonic cultures, suggesting that the
level of expression of virulence factors is different depending of the growth mode. Colorimetric
detection on wound biofilms of prevalent pathogens (S. aureus, P. aeruginosa and E. faecalis) is
also demonstrated using an ex-vivo porcine skin model of burn wound infection.
Original language | English |
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Pages (from-to) | 14909-14919 |
Number of pages | 11 |
Journal | ACS Applied Materials and Interfaces |
Volume | 8 |
Issue number | 24 |
Early online date | 22 Oct 2015 |
DOIs | |
Publication status | Published - 22 Jun 2016 |
Keywords
- Bandages
- Biofilms
- Humans
- Hydrogel, Polyethylene Glycol Dimethacrylate
- Pseudomonas aeruginosa
- Staphylococcus aureus
- Wound Infection
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Dive into the research topics of 'Prototype development of the intelligent hydrogel wound dressing and its efficacy in the detection of model pathogenic wound biofilms'. Together they form a unique fingerprint.Projects
- 1 Finished
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Encapsulated Phage for Treatment of Burns and Wound Site Infections
Arnot, T. (PI)
Engineering and Physical Sciences Research Council
2/05/11 → 1/05/15
Project: Research council
Profiles
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Toby Jenkins
Person: Research & Teaching, Affiliate staff
Equipment
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Confocal Laser Scanning Confocal Microscope (LSM)
Material and Chemical Characterisation (MC2)Facility/equipment: Equipment