Proteins of the pollen coat of Brassica oleracea

R. K. Ruiter; T. Mettenmeyer; D. Van Laarhoven; G. J. Van Eldik; J. Doughty; M. M.A. Van Herpen; J. A.M. Schrauwen; H. G. Dickinson; G. J. Wullems

doi:10.1016/S0176-1617(97)80185-X

Proteins of the pollen coat of Brassica oleracea

R. K. Ruiter, T. Mettenmeyer, D. Van Laarhoven, G. J. Van Eldik, J. Doughty, M. M.A. Van Herpen, J. A.M. Schrauwen, H. G. Dickinson, G. J. Wullems

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

14 Citations (SciVal)

Abstract

In higher plants, proteins in the coat of the pollen grain are assumed to play an important role in the interaction between pollen and stigma upon pollination. A polyclonal antiserum was raised against a mixture of these proteins. The antiserum strongly reacted with proteins extracted from the pollen coat and from whole stamens, whereas there was only a faint cross-reactivity to proteins from other tissues. Western blot analysis and immunolocalisation of pollen before and after rinsing with cyclohexane, a treatment that selectively removes the coat layer, showed that the proteins were exclusively located in the pollen coat. The same proved to hold for pollen coat proteins from other Brassica species. The availability of an antiserum creates the opportunity to identify coat protein encoding sequences in a cDNA library of anthers.

Original language	English
Pages (from-to)	85-91
Number of pages	7
Journal	Journal of Plant Physiology
Volume	150
Issue number	1-2
DOIs	https://doi.org/10.1016/S0176-1617(97)80185-X
Publication status	Published - 31 Dec 1997

Funding

The authors thank Dr. A. F. Croes for critically reading and discussing the manuscript. We arc grateful to G. van der Weerden and W. van den Brink for continuously maintaining our plant material, and Dr. M. Trick for supplying Brassica alboglabra plants. Dr. H. M. P. Kcngen and H. Geurts are thanked for technical assistence during electron microscopical investigations. This research was supported by funds of ~e BRIDGE programme of the Commission of the European Communities (Contract BlOT 900172).

Funders	Funder number
Commission of the European Communities	BlOT 900172

Keywords

Brassica oleracea
immunolocalisation
pollen coat proteins
western blotting

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/S0176-1617(97)80185-X

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title = "Proteins of the pollen coat of Brassica oleracea",

abstract = "In higher plants, proteins in the coat of the pollen grain are assumed to play an important role in the interaction between pollen and stigma upon pollination. A polyclonal antiserum was raised against a mixture of these proteins. The antiserum strongly reacted with proteins extracted from the pollen coat and from whole stamens, whereas there was only a faint cross-reactivity to proteins from other tissues. Western blot analysis and immunolocalisation of pollen before and after rinsing with cyclohexane, a treatment that selectively removes the coat layer, showed that the proteins were exclusively located in the pollen coat. The same proved to hold for pollen coat proteins from other Brassica species. The availability of an antiserum creates the opportunity to identify coat protein encoding sequences in a cDNA library of anthers.",

keywords = "Brassica oleracea, immunolocalisation, pollen coat proteins, western blotting",

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T1 - Proteins of the pollen coat of Brassica oleracea

AU - Ruiter, R. K.

AU - Mettenmeyer, T.

AU - Van Laarhoven, D.

AU - Van Eldik, G. J.

AU - Doughty, J.

AU - Van Herpen, M. M.A.

AU - Schrauwen, J. A.M.

AU - Dickinson, H. G.

AU - Wullems, G. J.

PY - 1997/12/31

Y1 - 1997/12/31

N2 - In higher plants, proteins in the coat of the pollen grain are assumed to play an important role in the interaction between pollen and stigma upon pollination. A polyclonal antiserum was raised against a mixture of these proteins. The antiserum strongly reacted with proteins extracted from the pollen coat and from whole stamens, whereas there was only a faint cross-reactivity to proteins from other tissues. Western blot analysis and immunolocalisation of pollen before and after rinsing with cyclohexane, a treatment that selectively removes the coat layer, showed that the proteins were exclusively located in the pollen coat. The same proved to hold for pollen coat proteins from other Brassica species. The availability of an antiserum creates the opportunity to identify coat protein encoding sequences in a cDNA library of anthers.

AB - In higher plants, proteins in the coat of the pollen grain are assumed to play an important role in the interaction between pollen and stigma upon pollination. A polyclonal antiserum was raised against a mixture of these proteins. The antiserum strongly reacted with proteins extracted from the pollen coat and from whole stamens, whereas there was only a faint cross-reactivity to proteins from other tissues. Western blot analysis and immunolocalisation of pollen before and after rinsing with cyclohexane, a treatment that selectively removes the coat layer, showed that the proteins were exclusively located in the pollen coat. The same proved to hold for pollen coat proteins from other Brassica species. The availability of an antiserum creates the opportunity to identify coat protein encoding sequences in a cDNA library of anthers.

KW - Brassica oleracea

KW - immunolocalisation

KW - pollen coat proteins

KW - western blotting

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DO - 10.1016/S0176-1617(97)80185-X

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SN - 0176-1617

VL - 150

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EP - 91

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 1-2

ER -

Proteins of the pollen coat of Brassica oleracea

Abstract

Funding

Keywords

ASJC Scopus subject areas

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