Projects per year
Abstract
Phosphorylation is the most important post-translational modification of proteins in eukaryotic cells and it is catalysed by enzymes called kinases. The balance between protein phosphorylation and dephosphorylation is critical for the regulation of physiological processes and its unbalance is the cause of several diseases. Conventional assays used to analyse the kinase activity are limited as they rely heavily on phospho-specific antibodies and radioactive tags. This makes their use impractical for high throughput drug discovery platforms. We have developed two versatile methods to detect the release of protons (H+) associated with the protein phosphorylation catalysed by kinases. The first approach is based on the pH-sensitive response of oxide-semiconductor interfaces and the second method detects the pH changes in phosphorylation reaction using a commercial micro pH electrode. The proposed methods successfully detected phosphorylation of myelin basic protein by PKC- kinase. These techniques can be readily adopted for multiplexed arrays and high throughput analysis of kinase activity, which will represent an important innovation in biomedical research and drug discovery.
Original language | English |
---|---|
Pages (from-to) | 109-114 |
Number of pages | 6 |
Journal | Biosensors and Bioelectronics |
Volume | 54 |
Early online date | 31 Oct 2013 |
DOIs | |
Publication status | Published - 15 Apr 2014 |
Keywords
- protein phosphorylation
- pH sensors
- drug discovery
Fingerprint
Dive into the research topics of 'Protein phosphorylation analysis based on proton release detection: potential tools for drug discovery'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Paracrine Regulation of Endothelial Cell Motility
Pula, G.
Biotechnology and Biological Sciences Research Council
15/05/12 → 14/05/15
Project: Research council
Profiles
-
Mirella Di Lorenzo
- Department of Chemical Engineering - Professor
- Centre for Sustainable and Circular Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Biosensors, Bioelectronics and Biodevices (C3Bio)
- Centre for Integrated Bioprocessing Research (CIBR)
- Faculty of Engineering and Design - Associate Dean (International)
Person: Research & Teaching
-
Pedro Estrela
- Department of Electronic & Electrical Engineering - Professor
- Centre for Biosensors, Bioelectronics and Biodevices (C3Bio)
- Centre for Therapeutic Innovation
- Centre for Sustainable and Circular Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Nanoscience and Nanotechnology
Person: Research & Teaching