Abstract

A method of improving the folding of an enzyme comprising a thiamine pyrophosphate (TPP) binding domain, the method comprising: providing a nucleic acid encoding the enzyme comprising a TPP binding domain, in which one or more of the TPP binding domains in the enzyme monomer are replaced with a TPP binding domain from a thermostable TPP-binding protein, and expressing the nucleic acid under conditions that allow expression and folding of the enzyme. The enzyme may be pyruvate decarboxylase.
Original languageEnglish
Patent numberWO 2013/175190 (BR112014029129, EP2852663, US2015104850)
Priority date22/05/12
Filing date20/05/13
Publication statusPublished - 2019

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