Abstract
Here, we report a new pentafluoropropanamido rhodamine fluorescent probe (ACS-HNE) that allows for the selective detection of neutrophil elastase (NE). ACS-HNE displayed high sensitivity, with a low limit of detection (<5.3 nM), and excellent selectivity toward elastase over other relevant biological analytes and enzymes. The comparatively poor solubility and cell permeability of neat ACS-HNE was improved by creating an ACS-HNE-albumin complex; this approach allowed for improvements in the in situ visualization of elastase activity in RAW 264.7 cells relative to ACS-HNE alone. The present study thus serves to demonstrate a simple universal strategy that may be used to overcome cell impermeability and solubility limitations, and to prepare probes suitable for the cellular imaging of enzymatic activity in vitro.
Original language | English |
---|---|
Article number | 389 |
Journal | Frontiers in Chemistry |
Volume | 8 |
DOIs | |
Publication status | Published - 3 Jun 2020 |
Keywords
- BSA-based nanocarrier
- cell imaging
- elastase detection
- fluorescence imaging
- nanocarrier-based enzyme detection
ASJC Scopus subject areas
- General Chemistry