Protein dynamics revealed by CPMG dispersion

Rieko Ishima, Stefan Bagby

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Since internal motion is essential to enable biomolecular function, it is important to understand the internal motion of proteins by applying biophysical methods. Nuclear magnetic resonance (NMR) methods can be used to probe protein dynamics in a nucleus-specific manner covering motions that occur over a wide range of time scales from the picosecond-nanosecond range to greater than seconds. Among these NMR methods, constant-time (CT) Carr-Purcell-Meiboom-Gill (CPMG) dispersion experiments are often applied to characterize protein equilibrium conformations that interconvert on the microsecond to millisecond time scale through the chemical exchange contribution to the detected transverse relaxation rates. This review covers developments and practical aspects of 15N-backbone CT-CPMG dispersion experiments that are frequently used for biomolecular studies, followed by 13C-methyl CT-CPMG dispersion experiments that are suitable for studies of proteins or assemblies in excess of about 50 kDa. Finally, for the efficient application of CPMG dispersion experiments, other NMR experiments that may be useful to cover a wider range of protein dynamics and so permit a more informed implementation and interpretation of CPMG dispersion experiments.
Original languageEnglish
Title of host publicationModern Magnetic Resonance, 2nd edition
EditorsGraham Webb
Place of Publication9783319283876
PublisherSpringer
Pages435-452
DOIs
Publication statusPublished - Jul 2017

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gills
nuclear magnetic resonance spectroscopy
proteins
methodology

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Ishima, R., & Bagby, S. (2017). Protein dynamics revealed by CPMG dispersion. In G. Webb (Ed.), Modern Magnetic Resonance, 2nd edition (pp. 435-452). 9783319283876: Springer. https://doi.org/10.1007/978-3-319-28388-3_44

Protein dynamics revealed by CPMG dispersion. / Ishima, Rieko; Bagby, Stefan.

Modern Magnetic Resonance, 2nd edition. ed. / Graham Webb. 9783319283876 : Springer, 2017. p. 435-452.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ishima, R & Bagby, S 2017, Protein dynamics revealed by CPMG dispersion. in G Webb (ed.), Modern Magnetic Resonance, 2nd edition. Springer, 9783319283876, pp. 435-452. https://doi.org/10.1007/978-3-319-28388-3_44
Ishima R, Bagby S. Protein dynamics revealed by CPMG dispersion. In Webb G, editor, Modern Magnetic Resonance, 2nd edition. 9783319283876: Springer. 2017. p. 435-452 https://doi.org/10.1007/978-3-319-28388-3_44
Ishima, Rieko ; Bagby, Stefan. / Protein dynamics revealed by CPMG dispersion. Modern Magnetic Resonance, 2nd edition. editor / Graham Webb. 9783319283876 : Springer, 2017. pp. 435-452
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