The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Lamble, H. J., Theodossis, A., Milburn, C. C., Taylor, G. L., Bull, S., Hough, D. W., & Danson, M. J. (2005). Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus. FEBS Letters, 579(30), 6865-6869. https://doi.org/10.1016/j.febslet.2005.11.028