Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus

H J Lamble, A Theodossis, C C Milburn, G L Taylor, Steven Bull, D W Hough, M J Danson

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Abstract

The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)6865-6869
Number of pages5
JournalFEBS Letters
Volume579
Issue number30
DOIs
Publication statusPublished - 19 Dec 2005

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Sulfolobus solfataricus
Archaea
Metabolism
Galactose
Glucose
Fructose-Bisphosphate Aldolase
Sugars
Kinetics
Substrates
Enzymes

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Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus. / Lamble, H J; Theodossis, A; Milburn, C C; Taylor, G L; Bull, Steven; Hough, D W; Danson, M J.

In: FEBS Letters, Vol. 579, No. 30, 19.12.2005, p. 6865-6869.

Research output: Contribution to journalArticle

Lamble, H J ; Theodossis, A ; Milburn, C C ; Taylor, G L ; Bull, Steven ; Hough, D W ; Danson, M J. / Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus. In: FEBS Letters. 2005 ; Vol. 579, No. 30. pp. 6865-6869.
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