Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai

Daisuke Saito, Chikako Morinaga, Yumiko Aoki, Shuhei Nakamura, Hiroshi Mitani, Makoto Furutani-Seiki, Hisato Kondoh, Minoru Tanaka

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The proliferation of germ cells becomes sexually dimorphic during gonadal sex differentiation, although the underlying dynamics of this are not well understood in vertebrates. By tracing GFP-labeled germ cells in vivo and analyzing the germ cell-depleted mutant, zenzai, we show that the proliferation and differentiation of germ cells are regulated in a sexually dimorphic manner in the teleost fish medaka. In the undifferentiated gonads, germ cells resume proliferation by slow intermittent division (type I), producing isolated daughter cells. While germ cells in the male gonads continue this mode of proliferation, some germ cell fractions in the female gonads initiate two to four rounds of continuous division (type II), forming cysts of four, eight, or sixteen cells, which subsequently enter meiosis synchronously. Thus, female germ cells become differentiated much earlier than do male germ cells. In the zenzai mutant, a defect in slow intermittent division eventually leads to the depletion of germ cells in the adult gonads in both sexes, despite the fact that cyst-forming division is unaffected. This argues that slow intermittent division is essential for the maintenance of germ cells. The proliferation and differentiation of germ cells are thus important components of gonadal sex differentiation in vertebrates.
Original languageEnglish
Pages (from-to)280-290
Number of pages11
JournalDevelopmental Biology
Volume310
Issue number2
DOIs
Publication statusPublished - 15 Oct 2007

Fingerprint

Oryzias
Sex Differentiation
Germ Cells
Gonads
Vertebrates
Cysts
Meiosis

Cite this

Saito, D., Morinaga, C., Aoki, Y., Nakamura, S., Mitani, H., Furutani-Seiki, M., ... Tanaka, M. (2007). Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai. Developmental Biology, 310(2), 280-290. https://doi.org/10.1016/j.ydbio.2007.07.039

Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai. / Saito, Daisuke; Morinaga, Chikako ; Aoki, Yumiko ; Nakamura, Shuhei; Mitani, Hiroshi ; Furutani-Seiki, Makoto; Kondoh, Hisato; Tanaka, Minoru.

In: Developmental Biology, Vol. 310, No. 2, 15.10.2007, p. 280-290.

Research output: Contribution to journalArticle

Saito, D, Morinaga, C, Aoki, Y, Nakamura, S, Mitani, H, Furutani-Seiki, M, Kondoh, H & Tanaka, M 2007, 'Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai', Developmental Biology, vol. 310, no. 2, pp. 280-290. https://doi.org/10.1016/j.ydbio.2007.07.039
Saito, Daisuke ; Morinaga, Chikako ; Aoki, Yumiko ; Nakamura, Shuhei ; Mitani, Hiroshi ; Furutani-Seiki, Makoto ; Kondoh, Hisato ; Tanaka, Minoru. / Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai. In: Developmental Biology. 2007 ; Vol. 310, No. 2. pp. 280-290.
@article{961f744a8dc549528e652a050a472e1d,
title = "Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai",
abstract = "The proliferation of germ cells becomes sexually dimorphic during gonadal sex differentiation, although the underlying dynamics of this are not well understood in vertebrates. By tracing GFP-labeled germ cells in vivo and analyzing the germ cell-depleted mutant, zenzai, we show that the proliferation and differentiation of germ cells are regulated in a sexually dimorphic manner in the teleost fish medaka. In the undifferentiated gonads, germ cells resume proliferation by slow intermittent division (type I), producing isolated daughter cells. While germ cells in the male gonads continue this mode of proliferation, some germ cell fractions in the female gonads initiate two to four rounds of continuous division (type II), forming cysts of four, eight, or sixteen cells, which subsequently enter meiosis synchronously. Thus, female germ cells become differentiated much earlier than do male germ cells. In the zenzai mutant, a defect in slow intermittent division eventually leads to the depletion of germ cells in the adult gonads in both sexes, despite the fact that cyst-forming division is unaffected. This argues that slow intermittent division is essential for the maintenance of germ cells. The proliferation and differentiation of germ cells are thus important components of gonadal sex differentiation in vertebrates.",
author = "Daisuke Saito and Chikako Morinaga and Yumiko Aoki and Shuhei Nakamura and Hiroshi Mitani and Makoto Furutani-Seiki and Hisato Kondoh and Minoru Tanaka",
year = "2007",
month = "10",
day = "15",
doi = "10.1016/j.ydbio.2007.07.039",
language = "English",
volume = "310",
pages = "280--290",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Elsevier Academic Press Inc",
number = "2",

}

TY - JOUR

T1 - Proliferation of germ cells during gonadal sex differentiation in medaka: Insights from germ cell-depleted mutant zenzai

AU - Saito, Daisuke

AU - Morinaga, Chikako

AU - Aoki, Yumiko

AU - Nakamura, Shuhei

AU - Mitani, Hiroshi

AU - Furutani-Seiki, Makoto

AU - Kondoh, Hisato

AU - Tanaka, Minoru

PY - 2007/10/15

Y1 - 2007/10/15

N2 - The proliferation of germ cells becomes sexually dimorphic during gonadal sex differentiation, although the underlying dynamics of this are not well understood in vertebrates. By tracing GFP-labeled germ cells in vivo and analyzing the germ cell-depleted mutant, zenzai, we show that the proliferation and differentiation of germ cells are regulated in a sexually dimorphic manner in the teleost fish medaka. In the undifferentiated gonads, germ cells resume proliferation by slow intermittent division (type I), producing isolated daughter cells. While germ cells in the male gonads continue this mode of proliferation, some germ cell fractions in the female gonads initiate two to four rounds of continuous division (type II), forming cysts of four, eight, or sixteen cells, which subsequently enter meiosis synchronously. Thus, female germ cells become differentiated much earlier than do male germ cells. In the zenzai mutant, a defect in slow intermittent division eventually leads to the depletion of germ cells in the adult gonads in both sexes, despite the fact that cyst-forming division is unaffected. This argues that slow intermittent division is essential for the maintenance of germ cells. The proliferation and differentiation of germ cells are thus important components of gonadal sex differentiation in vertebrates.

AB - The proliferation of germ cells becomes sexually dimorphic during gonadal sex differentiation, although the underlying dynamics of this are not well understood in vertebrates. By tracing GFP-labeled germ cells in vivo and analyzing the germ cell-depleted mutant, zenzai, we show that the proliferation and differentiation of germ cells are regulated in a sexually dimorphic manner in the teleost fish medaka. In the undifferentiated gonads, germ cells resume proliferation by slow intermittent division (type I), producing isolated daughter cells. While germ cells in the male gonads continue this mode of proliferation, some germ cell fractions in the female gonads initiate two to four rounds of continuous division (type II), forming cysts of four, eight, or sixteen cells, which subsequently enter meiosis synchronously. Thus, female germ cells become differentiated much earlier than do male germ cells. In the zenzai mutant, a defect in slow intermittent division eventually leads to the depletion of germ cells in the adult gonads in both sexes, despite the fact that cyst-forming division is unaffected. This argues that slow intermittent division is essential for the maintenance of germ cells. The proliferation and differentiation of germ cells are thus important components of gonadal sex differentiation in vertebrates.

UR - http://dx.doi.org/10.1016/j.ydbio.2007.07.039

U2 - 10.1016/j.ydbio.2007.07.039

DO - 10.1016/j.ydbio.2007.07.039

M3 - Article

VL - 310

SP - 280

EP - 290

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -