Prognostic value of an activation state marker for epidermal growth factor receptor in tissue microarrays of head and neck cancer

Anthony Kong, Pierre Leboucher, Russell Leek, Véronique Calleja, Stuart Winter, Adrian Harris, Peter J. Parker, Banafshé Larijani

Research output: Contribution to journalArticlepeer-review

Abstract

Overexpression and mutation of epidermal growth factor receptors (EGFR) have been shown to be important in the prognosis of several cancers, including head and neck cancers. However, our inability to define the activation status of these and other receptors limits our ability to assess the importance of these pathways and to exploit effectively new molecularly targeted treatments directed at their catalytic activities. Here we describe the use of automated, high-throughput fluorescence lifetime imaging microscopy to measure EGFR autophosphorylation status by fluorescence resonance energy transfer (FRET) in head and neck tumors. We have correlated FRET efficiency with the clinical and survival data. The results from head and neck arrays show that high FRET efficiency is correlated with worsening disease-free survival but not with overall survival. This powerful tool could be exploited as a new independent quantitative prognostic factor in clinical decisions and cancer management.

Original languageEnglish
Pages (from-to)2834-2843
Number of pages10
JournalCancer Research
Volume66
Issue number5
DOIs
Publication statusPublished - 1 Mar 2006

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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