Production of Protein Kinases in E. coli

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2 Citations (Scopus)


Recombinant protein expression is widely used to generate milligram quantities of protein kinases for crystallographic, enzymatic, or other biophysical assays in vitro. Expression in E. coli is fast, cheap, and reliable. Here I present a detailed protocol for the production of human Aurora-A kinase. I begin with transformation of a suitable plasmid into an expression strain of E. coli, followed by growth and harvesting of bacterial cell cultures. Finally, I describe the purification of Aurora-A to homogeneity using immobilized metal affinity and size exclusion chromatographies.
Original languageEnglish
Title of host publicationHeterologous Gene Expression in E. coli
EditorsN. Burgess-Brown
Place of PublicationNew York
Number of pages14
ISBN (Print)978-1-4939-6885-5
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, New York

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