Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment

Rakesh Bhatia; Jai B Lad; Maurice Bosch; David N Bryant; David Leak; Jason P Hallett; Telma T Franco; Joe A Gallagher

doi:10.1016/j.biortech.2020.124625

Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment

Rakesh Bhatia, Jai B Lad, Maurice Bosch, David N Bryant, David Leak, Jason P Hallett, Telma T Franco, Joe A Gallagher

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Abstract

Pretreatment strategies are fundamental to effectively deconstruct lignocellulosic biomass and economically produce biofuels, biomaterials and bio-based chemicals. This study evaluated individual and combinatorial steam explosion (SE) and ionic liquid (IL) pretreatments for production of high-value oligosaccharides from a novel seed-based Miscanthus hybrid (Mx2779). The two ILs used for pretreatment were triethylammonium hydrogen sulphate [TEA][HSO4] and 1-ethyl-3-methylimidazolium acetate [C2mim][OAc]. The results showed that each pretreatment leads to distinct effects on the fragmentation (cellulose and xylan dissolution, delignification, deacetylation) and physicochemical modification (cellulose and lignin properties) of lignocellulose. This, in turn, dictated enzymatic hydrolysis efficiencies of the cellulose pulp to glucose or gluco-oligosaccharides for downstream applications. Our findings suggest that the stand-alone SE or [C2mim][OAc] pretreatments may offer cost advantages over [TEA][HSO4] through the production of oligosaccharides such as xylo- and gluco-oligosaccharides. This study also highlights technical and economic pretreatment process challenges related to the production of oligosaccharides from Miscanthus Mx2779 biomass.

Original language	English
Article number	124625
Journal	Bioresource Technology
Volume	323
Early online date	28 Dec 2020
DOIs	https://doi.org/10.1016/j.biortech.2020.124625
Publication status	Published - 31 Mar 2021

Funding

The Biotechnology and Biological Sciences Research Council (BBSRC) ( BB/P017460/1 ) and the São Paulo Research Foundation (FAPESP) ( 2015/50612-8 ) financed this work. The authors thank John Clifton-Brown and the Miscanthus breeding team at IBERS for the Mx2779 hybrid (BBS/E/W/0012843A, BB/N016149/1, BB/K01711X/1, BBS/E/W/10963A01 as well as the GIANT-LINK project LK0863) and the BEACON Biorefining Centre of Excellence for supportive work. The Biotechnology and Biological Sciences Research Council (BBSRC) (BB/P017460/1) and the S?o Paulo Research Foundation (FAPESP) (2015/50612-8) financed this work. The authors thank John Clifton-Brown and the Miscanthus breeding team at IBERS for the Mx2779 hybrid (BBS/E/W/0012843A, BB/N016149/1, BB/K01711X/1, BBS/E/W/10963A01 as well as the GIANT-LINK project LK0863) and the BEACON Biorefining Centre of Excellence for supportive work.

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10.1016/j.biortech.2020.124625Licence: CC BY

An Integrated Approach to Explore a Novel Paradigm for Biofuel Production from Lignocellulosic Feedstocks
Arnot, T. (PI), Leak, D. (PI), Coma Bech, M. (CoI), Crennell, S. (CoI) & Henk, D. (CoI)
Biotechnology and Biological Sciences Research Council
1/03/17 → 31/03/22
Project: Research council

Cite this

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title = "Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment",

abstract = "Pretreatment strategies are fundamental to effectively deconstruct lignocellulosic biomass and economically produce biofuels, biomaterials and bio-based chemicals. This study evaluated individual and combinatorial steam explosion (SE) and ionic liquid (IL) pretreatments for production of high-value oligosaccharides from a novel seed-based Miscanthus hybrid (Mx2779). The two ILs used for pretreatment were triethylammonium hydrogen sulphate [TEA][HSO4] and 1-ethyl-3-methylimidazolium acetate [C2mim][OAc]. The results showed that each pretreatment leads to distinct effects on the fragmentation (cellulose and xylan dissolution, delignification, deacetylation) and physicochemical modification (cellulose and lignin properties) of lignocellulose. This, in turn, dictated enzymatic hydrolysis efficiencies of the cellulose pulp to glucose or gluco-oligosaccharides for downstream applications. Our findings suggest that the stand-alone SE or [C2mim][OAc] pretreatments may offer cost advantages over [TEA][HSO4] through the production of oligosaccharides such as xylo- and gluco-oligosaccharides. This study also highlights technical and economic pretreatment process challenges related to the production of oligosaccharides from Miscanthus Mx2779 biomass.",

author = "Rakesh Bhatia and Lad, {Jai B} and Maurice Bosch and Bryant, {David N} and David Leak and Hallett, {Jason P} and Franco, {Telma T} and Gallagher, {Joe A}",

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doi = "10.1016/j.biortech.2020.124625",

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AU - Bhatia, Rakesh

AU - Lad, Jai B

AU - Bosch, Maurice

AU - Bryant, David N

AU - Leak, David

AU - Hallett, Jason P

AU - Franco, Telma T

AU - Gallagher, Joe A

PY - 2021/3/31

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AB - Pretreatment strategies are fundamental to effectively deconstruct lignocellulosic biomass and economically produce biofuels, biomaterials and bio-based chemicals. This study evaluated individual and combinatorial steam explosion (SE) and ionic liquid (IL) pretreatments for production of high-value oligosaccharides from a novel seed-based Miscanthus hybrid (Mx2779). The two ILs used for pretreatment were triethylammonium hydrogen sulphate [TEA][HSO4] and 1-ethyl-3-methylimidazolium acetate [C2mim][OAc]. The results showed that each pretreatment leads to distinct effects on the fragmentation (cellulose and xylan dissolution, delignification, deacetylation) and physicochemical modification (cellulose and lignin properties) of lignocellulose. This, in turn, dictated enzymatic hydrolysis efficiencies of the cellulose pulp to glucose or gluco-oligosaccharides for downstream applications. Our findings suggest that the stand-alone SE or [C2mim][OAc] pretreatments may offer cost advantages over [TEA][HSO4] through the production of oligosaccharides such as xylo- and gluco-oligosaccharides. This study also highlights technical and economic pretreatment process challenges related to the production of oligosaccharides from Miscanthus Mx2779 biomass.

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JO - Bioresource Technology

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Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment

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An Integrated Approach to Explore a Novel Paradigm for Biofuel Production from Lignocellulosic Feedstocks

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