Product diversity linked to substrate usage in chain elongation by mixed-culture fermentation

Marta Coma, Ramiro Vilchez-Vargas, Hugo Roume, Ruy Jauregui, Dietmar H. Pieper, Korneel Rabaey

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)
232 Downloads (Pure)

Abstract

Acetate and ethanol can be converted to caproic acid by microorganisms through reverse β-oxidation. There is limited insight into the versatility of chain elongation in view of different starting substrates, including even- and odd-carbon carboxylates and alcohols other than ethanol. Thermodynamic analyses show that most elongation pathways are energetically feasible. Through incubations of microbial communities with different substrate-pair combinations, we established that ethanol and propanol were both highly suitable for chain elongation. As an electron acceptor, acetate, propionate, and butyrate readily elongated with ethanol, whereas an adaptation period was necessary for formate. Isobutyrate and longer-chained fatty acids above butyrate were not elongated. The microbial communities converged, and consistent enrichment of Clostridium spp. was observed, independent of the supplied alcohol or carboxylate, with a strain related to Clostridium kluyveri dominating the enrichments. Community analysis also showed phylotypes related to acteroidaceae and Microbacteriaceae families in all tests that are capable of converting the base substrates to useful intermediates. These organisms were mainly enriched with methanol or formate. Our overall conclusion is thus that multiple substrates can be used for chain elongation and that this process is carried out by highly similar organisms for direct chain elongation irrespective of the substrate.
Original languageEnglish
Pages (from-to)6467-6476
Number of pages10
JournalEnvironmental Science & Technology
Volume50
Issue number12
Early online date10 May 2016
DOIs
Publication statusPublished - 21 Jun 2016

Keywords

  • alcohols
  • Fermentation
  • medium chain fatty acids
  • mixed culture
  • reversed beta oxidation

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