Abstract
This is the first study to provide a preliminary investigation into the recovery of protein from wheat germ and the prediction of their extraction conditions in microwave cavity using a novel DES solvent. The response surface methodology was used to predict the microwaved protein extraction conditions of the DWG. The effects of DES buffer-concentration (X 1: 0.01-1.00 g/mL), microwave power (X 2: 50-250 W), irradiation time (X 3: 1-5 min) and sample-to-buffer ratio (X 4: 1:10-1:50) were tested using a single factor and Box-Behnken experimental design. Under the optimized conditions (X 1 = 0.52 g/mL, X 2 = 186 W, X 3 = 3.28 min, and X 4 = 1:39) protein yield and absorbed microwave were obtained at the optimal value of 33.00 % and 677 J/min, respectively with no denaturation of the protein as validated from the SDS-PAGE gel electrophoresis profile. Consequently, this investigation provides a practical approach for the extraction of bioactive protein from DWG using a novel deep eutectic solvent.
Original language | English |
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Article number | 135224 |
Journal | Food Chemistry |
Volume | 409 |
Issue number | 135224 |
Early online date | 16 Dec 2022 |
DOIs | |
Publication status | Published - 30 May 2023 |
Keywords
- Deep eutectic solvent (DES)
- Defatted wheat germs
- Microwave extraction
- Optimization
- Protein
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science