Probing the tri-trophic interaction between insects, nematodes and Photorhabdus

I Eleftherianos, S Joyce, R H Ffrench-Constant, D J Clarke, Stuart E Reynolds

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Photorhabdus sp. are entomopathogenic bacteria which, upon experimental infection, interact with the insect immune system, but little is known about the roles of their symbiotic nematode partners Heterorhabditis sp. in natural infections. Here, we investigated the respective contributions of nematodes and bacteria by examining humoral and cellular immune reactions of the model lepidopteran insect Manduca sexta against Heterorhabditis carrying Photorhabdus, nematodes free of bacteria (axenic nematodes) and bacteria alone. Insect mortality was slower following infection with axenic nematodes than when insects were infected with nematodes containing Photorhabdus, or the bacteria alone. Nematodes elicited host immune responses to a lesser extent than bacteria. Transcription of certain recognition and antibacterial genes was lower when insects were naturally infected with nematodes carrying no bacteria compared to insects that received bacteria, either with or without nematodes. Axenic nematodes also did not elicit such high levels of phenoloxidase activity and haemocyte aggregates as did treatments involving Photorhabdus. By contrast, the phagocytic capability of host haemocytes was decreased by both axenic and bacteria-associated nematodes, but not by Photorhabdus alone. These results imply that both bacteria and nematodes contribute separately to the pathogenic modulation of host immune responses during natural infections by the mutualistic Heterorhabdus-Photorhabdus complex.
Original languageEnglish
Pages (from-to)1695-1706
Number of pages12
JournalParasitology
Volume137
Issue number11
DOIs
Publication statusPublished - Sep 2010

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Photorhabdus
entomopathogenic nematodes
Insects
Nematoda
Bacteria
bacteria
insects
Hemocytes
Heterorhabditis
Infection
hemocytes
infection
Manduca
entomopathogenic bacteria
immune response
Monophenol Monooxygenase
monophenol monooxygenase
Manduca sexta
cell aggregates
Immune System

Cite this

Eleftherianos, I., Joyce, S., Ffrench-Constant, R. H., Clarke, D. J., & Reynolds, S. E. (2010). Probing the tri-trophic interaction between insects, nematodes and Photorhabdus. Parasitology, 137(11), 1695-1706. https://doi.org/10.1017/s0031182010000508

Probing the tri-trophic interaction between insects, nematodes and Photorhabdus. / Eleftherianos, I; Joyce, S; Ffrench-Constant, R H; Clarke, D J; Reynolds, Stuart E.

In: Parasitology, Vol. 137, No. 11, 09.2010, p. 1695-1706.

Research output: Contribution to journalArticle

Eleftherianos, I, Joyce, S, Ffrench-Constant, RH, Clarke, DJ & Reynolds, SE 2010, 'Probing the tri-trophic interaction between insects, nematodes and Photorhabdus', Parasitology, vol. 137, no. 11, pp. 1695-1706. https://doi.org/10.1017/s0031182010000508
Eleftherianos, I ; Joyce, S ; Ffrench-Constant, R H ; Clarke, D J ; Reynolds, Stuart E. / Probing the tri-trophic interaction between insects, nematodes and Photorhabdus. In: Parasitology. 2010 ; Vol. 137, No. 11. pp. 1695-1706.
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