Prior exercise alters the difference between arterialised and venous glycaemia

implications for blood sampling procedures

Research output: Contribution to journalArticle

6 Citations (Scopus)
39 Downloads (Pure)

Abstract

Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmolL-1 respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044.
Original languageEnglish
Pages (from-to)1414-1421
Number of pages8
JournalBritish Journal of Nutrition
Volume117
Issue number10
DOIs
Publication statusPublished - 28 May 2017

Fingerprint

Exercise
Glucose Tolerance Test
Insulin Resistance
Glucose
Area Under Curve
Veins
Arm
Hand

Keywords

  • Adult
  • Blood Glucose
  • Blood Specimen Collection
  • Cross-Over Studies
  • Energy Metabolism
  • Exercise
  • Glucose Tolerance Test
  • Humans
  • Insulin Resistance
  • Male
  • Young Adult
  • Journal Article

Cite this

@article{5686bf445aff4a7e91c633e2e7b8e0df,
title = "Prior exercise alters the difference between arterialised and venous glycaemia: implications for blood sampling procedures",
abstract = "Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmolL-1 respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044.",
keywords = "Adult, Blood Glucose, Blood Specimen Collection, Cross-Over Studies, Energy Metabolism, Exercise, Glucose Tolerance Test, Humans, Insulin Resistance, Male, Young Adult, Journal Article",
author = "Robert Edinburgh and Aaron Hengist and Harry Smith and James Betts and Dylan Thompson and Jean-Philippe Walhin and Javier Gonzalez",
year = "2017",
month = "5",
day = "28",
doi = "10.1017/S0007114517001362",
language = "English",
volume = "117",
pages = "1414--1421",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "10",

}

TY - JOUR

T1 - Prior exercise alters the difference between arterialised and venous glycaemia

T2 - implications for blood sampling procedures

AU - Edinburgh, Robert

AU - Hengist, Aaron

AU - Smith, Harry

AU - Betts, James

AU - Thompson, Dylan

AU - Walhin, Jean-Philippe

AU - Gonzalez, Javier

PY - 2017/5/28

Y1 - 2017/5/28

N2 - Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmolL-1 respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044.

AB - Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmolL-1 respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044.

KW - Adult

KW - Blood Glucose

KW - Blood Specimen Collection

KW - Cross-Over Studies

KW - Energy Metabolism

KW - Exercise

KW - Glucose Tolerance Test

KW - Humans

KW - Insulin Resistance

KW - Male

KW - Young Adult

KW - Journal Article

UR - http://dx.doi.org/10.1017/S0007114517001362

U2 - 10.1017/S0007114517001362

DO - 10.1017/S0007114517001362

M3 - Article

VL - 117

SP - 1414

EP - 1421

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 10

ER -